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Published February 23, 2012 | Published
Book Section - Chapter Open

In vivo imaging of cell nuclei by photoacoustic microscopy without staining


Ultraviolet photoacoustic microscopy (UVPAM) can image cell nuclei in vivo with high contrast and resolution noninvasively without staining. Here, we used UV light at wavelengths of 210-310 nm for excitation of DNA and RNA to produce photoacoustic waves. We applied the UVPAM to in vivo imaging of cell nuclei in mouse skin, and obtained UVPAM images of the unstained cell nuclei at wavelengths of 245-282 nm as ultrasound gel was used for acoustic coupling. The largest ratio of contrast to noise was found for the images of cell nuclei at a 250 nm wavelength.

Additional Information

© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). This work was sponsored in part by National Institutes of Health grants R01 EB000712, R01 EB008085, R01 CA113453901, U54 CA136398, 5P60 DK02057933, and U54 CA136398. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

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