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Published March 1, 2016 | Published + Supplemental Material
Journal Article Open

Spatial Variations in Vitreous Oxygen Consumption


We investigated the spatial variation of vitreous oxygen consumption in enucleated porcine eyes. A custom made oxygen source was fabricated that could be localized to either the mid or posterior vitreous cavity and steady state vitreous oxygen tension was measured as a function of distance from the source using a commercially available probe. The reaction rate constant of ascorbate oxidation was estimated ex vivo by measuring the change in oxygen tension over time using vitreous harvested from porcine eyes. Vitreous ascorbate from mid and posterior vitreous was measured spectrophotometrically. When the oxygen source was placed in either the mid-vitreous (N = 6) or the posterior vitreous (N = 6), we measured a statistically significant decrease in vitreous oxygen tension as a function of distance from the oxygen source when compared to control experiments without an oxygen source; (p<0.005 for mid-vitreous and p<0.018 for posterior vitreous at all distances). The mid-vitreous oxygen tension change was significantly different from the posterior vitreous oxygen tension change at 2 and 3mm distances from the respective oxygen source (p<0.001). We also found a statistically significant lower concentration of ascorbate in the mid-vitreous as compared to posterior vitreous (p = 0.02). We determined the reaction rate constant, k = 1.61 M^(-1)s^(-1) ± 0.708 M^(-1)s^(-1) (SE), of the oxidation of ascorbate which was modeled following a second order rate equation. Our data demonstrates that vitreous oxygen consumption is higher in the posterior vitreous compared to the mid-vitreous. We also show spatial variations in vitreous ascorbate concentration.

Additional Information

© 2016 Murali et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received: September 23, 2015; Accepted: February 8, 2016; Published: March 1, 2016. The authors would like to thank the National Institutes of Health, Whittier Foundation, Harrington Foundation, USC Eye Institute, Institute of Biomedical Therapeutics, and the Research to Prevent Blindness. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors have declared that no competing interests exist. Author Contributions: Conceived and designed the experiments: KM DK HN NS EC YT MH AK. Performed the experiments: KM. Analyzed the data: KM DK HN NS EC YT MH AK. Contributed reagents/materials/analysis tools: DK NS YT. Wrote the paper: KM AK.

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Published - journal.pone.0149961.PDF

Supplemental Material - journal.pone.0149961.s001.DOCX


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