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Published 2010 | Supplemental Material + Published
Journal Article Open

A self-powered, one-step chip for rapid, quantitative and multiplexed detection of proteins from pinpricks of whole blood


We describe an automated, self-powered chip based on lateral flow immunoassay for rapid, quantitative, and multiplex protein detection from pinpricks of whole blood. The device incorporates on-chip purification of blood plasma by employing inertial forces to focus blood cells away from the assay surface, where plasma proteins are captured and detected on antibody "barcode" arrays. Power is supplied from the capillary action of a piece of adsorbent paper, and sequentially drives, over a 40 minute period, the four steps required to capture serum proteins and then develop a multiplex immunoassay. An 11 protein panel is assayed from whole blood, with high sensitivity and high reproducibility. This inexpensive, self-contained, and easy to operate chip provides a useful platform for point-of-care diagnoses, particularly in resource-limited settings.

Additional Information

© 2010 Royal Society of Chemistry. Received 19th June 2010; Accepted 9th September 2010. First published on the web 6th October 2010. This work was supported by the National Cancer Institute Grant No. 5U54 CA119347 (J.R.H. and P.I.), by a gift from the Jean Perkins Foundation and by the Ben & Catherine Ivy Foundation.

Attached Files

Published - Wang2010p11523Lab_on_a_Chip.pdf

Supplemental Material - C0LC00132E.PDF

Supplemental Material - SIVIDEO1.wmv

Supplemental Material - SIVIDEO2.wmv

Supplemental Material - SIVIDEO3.wmv

Supplemental Material - SIVIDEO4.wmv


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August 19, 2023
October 20, 2023