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Published May 23, 1986 | public
Journal Article

Detection of factors that interact with the human β-interferon regulatory region in vivo by DNAase I footprinting


We have used a DNAase I genomic footprinting procedure to detect interactions between cellular factors and the regulatory sequences of the human β-interferon gene. Prior to induction with poly(I)-poly(C), factors that bind to DNA are detected in one region located between −94 and −167 from the mRNA cap site, and in another region located between −68 and −38. After induction these factors dissociate and another factor binds to a region located between −77 and −64. Correlation of these footprints with the effects of deletions in the regulatory region of the β-interferon gene (accompanying paper) suggest that the factors that bind prior to induction are repressor molecules, while the component that binds after induction is a transcription factor. Dissociation of the repressor molecules from the DNA after induction may allow the transcription factor to bind to and activate the β-interferon promoter. Thus, the β-interferon gene may be controlled by a negative regulatory mechanism.

Additional Information

© 1986 Cell Press. Received 13 January 1986, Revised 17 March 1986. We thank George Church for invaluable help with the genomic sequencing technique, and Stephen Goodbourn, Anne Ephrussi, Tamar Enoch, Ann Hochschild, and Patrick Charnay for helpful discussions. This work was supported by a grant from the National Institutes of Health to T. M. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

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