DMD-based spatially Fourier-encoded photoacoustic microscopy
We present spatially Fourier-encoded photoacoustic microscopy using a digital micromirror device (DMD). The spatial fluence distribution of laser pulses is Fourier-encoded by the DMD, and a series of such encoded photoacoustic (PA) measurements enables decoding of the spatial distribution of optical absorption. By imaging a chromium target, we demonstrated the throughput and Fellgett advantages, which increased the PA signal-to-noise ratio (SNR) compared to raster scanning. The system was used to image two biological targets, a monolayer of red blood cells, and melanoma cells. The enhanced SNR benefited PA images by increasing the image's contrast-to-noise ratio and target identifiability.
© 2015 Society of Photo-Optical Instrumentation Engineers. The authors thank Dr. Lijun Ma, Yong Zhou, Dr. Chi Zhang, and Lei Li for helpful discussions, and Professor James Ballard for close reading of the manuscript. This work was sponsored in part by National Institutes of Health grants DP1 EB016986 (NIH Director's Pioneer Award), R01CA134539, R01CA157277, and R01 CA159959. L. W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
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