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Published April 28, 2021 | public
Journal Article Open

Spatial Control of Probiotic Bacteria in the Gastrointestinal Tract Assisted by Magnetic Particles


Engineered probiotics have the potential to diagnose and treat a variety of gastrointestinal (GI) diseases. However, these exogenous bacterial agents have limited ability to effectively colonize specific regions of the GI tract due to a lack of external control over their localization and persistence. Magnetic fields are well suited to providing such control, since they freely penetrate biological tissues. However, they are difficult to apply with sufficient strength to directly manipulate magnetically labeled cells in deep tissue such as the GI tract. Here, it is demonstrated that a composite biomagnetic material consisting of microscale magnetic particles and probiotic bacteria, when orally administered and combined with an externally applied magnetic field, enables the trapping and retention of probiotic bacteria within the GI tract of mice. This technology improves the ability of these probiotic agents to accumulate at specific locations and stably colonize without antibiotic treatment. By enhancing the ability of GI‐targeted probiotics to be at the right place at the right time, cellular localization assisted by magnetic particles (CLAMP) adds external physical control to an important emerging class of microbial theranostics.

Additional Information

© 2021 Wiley-VCH GmbH. Issue Online: 27 April 2021; Version of Record online: 11 March 2021; Manuscript revised: 19 January 2021; Manuscript received: 01 November 2020. M.T.B. and P.R. contributed equally to this work. This work was supported by the Pew Charitable Trust, the Packard Foundation, and the Heritage Medical Research Institute. M.T.B. was supported by an NSF GRFP fellowship. P.R. was supported by a Rosen center for Bioengineering predoctoral fellowship. The authors would like to thank Dr. Said Bogatyrev and Dr. Asher Preska Steinberg for their helpful advice, the Caltech Flow Cytometry Facility for assistance with flow cytometry, and Ivetta Vorobyova for her help with the X‐ray CT studies. The authors declare no conflict of interest. Data Availability Statement: The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Supplemental Material - adma202007473-sup-0001-suppmat.pdf


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August 20, 2023
August 20, 2023