1
M
aterials
D
esign
A
nalysis
R
eporting (MDAR)
Checklist for Authors
The MDAR framework establishes a minimum set of requirements in transparent reporting applicable to studies in the life scien
ces
(see Statement of Task:
doi:10.31222/osf.io/9sm4x.
). The
MDAR checklist is a tool for authors, editors
,
and others seeking to adopt
the MDAR framework for transparent reporting in manuscripts and other outputs. Please refer to the MDAR Elaboration Document
for additional context for the MDAR framework.
2
For
all that apply, please note where in the manuscript the required information is provided.
Materials
:
Newly created materials
indicate where provided: page no/section/legend)
n/a
The manuscript includes a dedicated "materials
availability statement" providing transparent
disclosure about availability of newly created
m
aterials including details on how materials
c
an be
accessed and describing any restrictions on access.
Data and mat
erials availability. P59
Antibodies
indicate where provided: page no/section/legend)
n/a
For commercial reagents, provide supplier name,
catalogue number and
RRID
, if available.
Methods pages 31
-
36
DNA and RNA sequences
indicate where provided: page no/section/legend)
n/a
Short novel
DNA or RNA
including primers, probes
:
Sequences
should be included or deposited in
a
public repository.
Table S5 and Table S6
Cell materials
indicate where provided: page no/section/legend
n/a
Cell lines:
Provide species information, strain.
P
rovide accession number in repository
OR
supplier
name, catalog number, clone number,
OR
RRID
.
x
Primary cultures:
Provide species, strain, sex of
origin, genetic modification statu
s.
C57Bl6 mice female, Crispr deletion mutants of Il2ra
Experimental animals
indicate where provided: page no/section/legend)
n/a
Laboratory animals
or Model organisms
:
Provide
species, strain, sex, age, genetic
modification status
.
Provide
a
ccession number in repository
OR
supplier
name, catalog number, clone number,
OR
RRID
.
C57Bl6 mice female
Methods p30
-
40
Animal observed in or captured from the field:
Provide species, sex
,
and age where possible
.
X
Plants and microbes
indicate where provided: page no/section/legend)
n/a
Plants:
provide species and strain,
ecotype and
cultivar where relevant
,
unique accession number if
available, and source (including location for collected
wild specimens)
.
X
Microbes:
provide species and strain, unique
accession number if available, and source
.
X
Human research participants
indicate where provided: page no/section/legend)
or
state if these demographics were not collected
n/a
If collected and within the
bounds of privacy
constraints r
eport on age
,
sex and gender
or
ethnicity
for all study participants.
x
3
Design
:
Study protocol
indicate where provided: page no/section/legend)
n/a
If study protocol has been pre
-
registered, provide
DOI.
For clinical trials, provide the trial registration
number
OR
cite DOI.
x
Laboratory protocol
indicate where provided: page no/section/legend)
n/a
Provide DOI
OR
other citation details if detailed step
-
by
-
step protocols are available.
x
Experimental study design (statistics details)
For in vivo studies
:
State whether and how the
following have been done
indicate where provided: page no/section/legend. If it
could have been done, but was not, write not done
n/a
Sample size
determination
x
Randomisation
x
Blinding
x
Inclusion/exclusion criteria
x
Sample definition and in
-
laboratory replication
indicate where provided: page no/section/legend
n/a
State number of times the experiment was
replicated in laboratory
.
Information provided in figure legends
Define whether data describe technical or biological
replicates
.
Data describes biological replicates
Ethics
indicate where provided: page no/section/legend
n/a
Studies involving human participants:
State details
of authority granting ethics approval (IRB or
equivalent committee(s), provide reference number
for approval.
x
Studies involving experimental animals:
State
details of authority granting ethics approval (IRB or
equivalent committee(s), provide reference number
for approval.
Approved by NHLBI Animal Care & Use Committee
Studies involving specimen and field samples:
State
if relevant permits obtained, provide details of
authority approving study; if none were required,
explain why.
x
Dual Use Research of Concern (DURC)
indicate where provided: page no/section/legend
n/a
If
study is subject to dual use research of
concern
regulations
, state
the authority granting approval
and reference number for the regulatory approval.
x
4
Analysis
:
Attrition
indicate where provided: page no/section/legend
n/a
Describe whether
exclusion criteria were
preestablished. Report if sample or data points were
omitted from analysis. If yes report if this was due to
attrition or intentional exclusion and provide
justification.
x
Statistics
indicate where provided: page
no/section/legend
n/a
Describe statistical tests used and justify choice of
tests.
Students t tests were performed as described in
legends.
Data
a
vailability
indicate where provided: page no/section/legend
n/a
For newly created and reused
datasets, the
manuscript includes a data availability statement
that provides details for access or notes restrictions
on access.
Data and material availability p. 59
If newly created
data
sets
are publicly available,
provide accession number in repository
OR
DOI
OR
URL and licensing details where available.
Information found in data and material availability
section
If
reused data is
publicly available provide accession
number in repository
OR
DOI
OR
URL,
OR
citation.
x
Code
a
vailability
indicate where provided: page no/section/legend
n/a
For all newly generated custom computer
code/software/mathematical algorithm or re
-
used
code essential for replicating the main findings of
the study, the manuscript includes a data availability
statement that provides details for access or notes
restrictions.
x
If newly generated code is publicly available, provide
accession number in
repository,
OR
DOI
OR
URL and
licensing details where available. State any
restrictions on code availability or accessibility
.
x
If reused code is
publicly available provide accession
number in repository
OR
DOI
OR
URL,
OR
citation.
x
5
Reporting
MDAR framework recommends adoption of discipline
-
specific guidelines, established
and endorsed through community
initiatives. Journals have their own policy about requiring specific guidelines and recommendations to complement MDAR.
Adherence to community standards
indicate where provided: page no/section/legend
n/a
State if relevant guidelines (e.g., ICMJE, MIBBI,
ARRIVE) have been followed, and whether a checklist
(e.g., CONSORT, PRISMA, ARRIVE) is provided with
the manuscript.
x