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Published March 3, 2014 | Published
Book Section - Chapter Open

Photothermal bleaching and recovery analysis in photoacoustic microscopy


A novel method – photoacoustic recovery after photothermal bleaching (PRAP) – is proposed and implemented to study particle dynamics and medium properties at the micron scale via photoacoustic imaging. PRAP is an intuitive way to visualize as well as quantify dynamic processes in many kinds of media. We demonstrate PRAP first in a phantom study, and then in live cells. PRAP provides high signal-to-noise ratio imaging with minimal bleaching-induced artifacts during the recovery stage, ideal for monitoring the diffusive and kinetic phenomena inside a cell.

Additional Information

© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE). We thank Ms. Seema Dahlheimer for her attentive reading of the manuscript. This work was sponsored in part by National Institutes of Health grants DP1 EB016986 (NIH Director's Pioneer Award), R01 CA134539, and R01 CA159959. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work. K.M. has a financial interest in Microphotoacoustics, Inc.

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