Supplemental data: 15N backbone relaxation values Included in the tables: R1: the longitudinal relaxation rate constant (1/s) R2o: the transverse relaxation rate constant that is free of conformational exchange contributions (1/s) NOE: steady-state 1H-15N NOE nxy and nz: the transverse and longitudinal cross-correlation relaxation rate constants, respectively Rex: the conformational exchange contribution to R2 (where R2 = Rex + R2o) (1/s) WT Gb1 (1.5 mM, pH 6, 25C) # R1 error R2o error NOE error nxy error nz error Rex error 1 2 2.358 0.036 4.539 0.114 0.769 0.004 3.181 0.007 1.503 0.007 3 2.311 0.054 4.563 0.165 0.753 0.003 3.148 0.016 1.518 0.007 4 2.459 0.060 4.661 0.182 0.862 0.012 3.305 0.010 1.606 0.012 5 2.545 0.046 4.647 0.156 0.890 0.007 3.502 0.019 1.713 0.010 6 2.528 0.044 4.712 0.162 0.891 0.001 3.427 0.025 1.615 0.008 7 2.262 0.072 4.249 0.233 0.803 0.006 3.218 0.031 1.601 0.013 8 2.287 0.009 4.347 0.067 0.828 0.002 3.260 0.006 1.531 0.012 9 2.375 0.020 4.466 0.095 0.805 0.003 2.806 0.015 1.362 0.007 10 2.504 0.058 4.562 0.169 0.868 0.005 3.083 0.008 1.506 0.010 11 2.699 0.084 5.225 0.252 0.782 0.003 2.750 0.030 1.383 0.005 12 2.288 0.094 4.323 0.289 0.571 0.002 2.671 0.016 1.344 0.017 13 2.179 0.056 4.160 0.152 0.726 0.002 2.924 0.008 1.483 0.008 14 2.026 0.014 3.923 0.068 0.699 0.002 2.495 0.012 1.232 0.005 15 2.318 0.016 4.651 0.061 0.615 0.001 2.928 0.003 1.418 0.006 0.553 0.131 16 2.466 0.041 5.032 0.086 0.795 0.002 3.026 0.006 1.483 0.009 0.075 0.086 17 2.827 0.106 5.330 0.305 0.702 0.001 3.048 0.022 1.459 0.007 18 2.479 0.020 4.591 0.091 0.796 0.006 3.121 0.016 1.502 0.006 19 2.362 0.008 4.758 0.089 0.704 0.002 3.184 0.010 1.507 0.016 20 2.686 0.077 4.587 0.242 0.877 0.003 3.289 0.010 1.595 0.018 21 2.455 0.012 4.265 0.063 0.663 0.001 2.983 0.007 1.414 0.006 22 2.163 0.060 4.320 0.184 0.786 0.006 3.076 0.013 1.477 0.011 23 2.725 0.072 5.857 0.218 0.756 0.002 3.444 0.009 1.548 0.009 24 2.389 0.023 5.138 0.113 0.702 0.005 3.717 0.011 1.611 0.011 25 2.882 0.019 5.911 0.099 0.889 0.010 3.536 0.016 1.582 0.006 26 2.648 0.028 5.151 0.138 0.872 0.001 3.749 0.015 1.768 0.017 27 2.602 0.080 5.428 0.264 0.832 0.057 3.595 0.011 1.560 0.012 28 2.202 0.099 4.914 0.290 0.872 0.004 3.708 0.010 1.631 0.015 29 2.435 0.034 4.855 0.166 0.933 0.008 3.635 0.017 1.657 0.020 30 2.360 0.046 4.967 0.189 0.917 0.004 3.599 0.019 1.603 0.017 31 2.388 0.069 5.073 0.224 0.779 0.015 3.996 0.012 1.737 0.012 32 2.417 0.063 5.022 0.225 0.923 0.054 3.923 0.025 1.704 0.012 33 2.359 0.012 4.644 0.115 0.920 0.007 3.814 0.014 1.688 0.021 34 2.378 0.018 4.911 0.079 0.914 0.006 3.892 0.014 1.739 0.006 35 2.313 0.026 4.878 0.136 0.903 0.011 3.710 0.018 1.591 0.014 36 2.255 0.051 4.970 0.191 0.906 0.005 3.604 0.013 1.593 0.017 37 2.631 0.021 5.058 0.080 0.696 0.002 3.397 0.008 1.639 0.006 38 2.342 0.026 4.490 0.099 0.894 0.005 3.564 0.012 1.663 0.008 39 2.349 0.073 4.837 0.249 0.876 0.003 3.598 0.019 1.614 0.017 40 1.960 0.023 4.177 0.090 0.682 0.005 3.181 0.007 1.456 0.009 0.052 0.177 41 1.637 0.031 3.196 0.160 0.384 0.002 1.955 0.022 0.956 0.013 0.222 0.227 42 2.369 0.041 4.237 0.153 0.905 0.002 3.322 0.015 1.575 0.013 43 2.188 0.013 4.191 0.097 0.652 0.031 3.146 0.024 1.602 0.009 0.153 0.238 44 2.467 0.059 4.592 0.164 0.749 0.007 3.105 0.013 1.508 0.004 45 2.527 0.043 5.250 0.143 0.896 0.005 3.086 0.009 1.474 0.010 0.422 0.309 46 2.599 0.032 4.643 0.151 0.846 0.001 3.435 0.023 1.681 0.015 47 2.697 0.015 5.509 0.082 0.816 0.007 3.073 0.006 1.484 0.010 0.542 0.189 48 2.275 0.038 4.844 0.151 0.702 0.002 3.271 0.013 1.501 0.013 0.556 0.233 49 2.850 0.056 5.371 0.287 0.779 0.004 3.089 0.051 1.501 0.017 50 3.036 0.104 4.838 0.267 0.957 0.003 3.729 0.016 1.835 0.008 51 3.055 0.121 5.200 0.313 0.869 0.058 3.111 0.018 1.504 0.003 52 2.571 0.053 4.841 0.184 0.861 0.007 3.201 0.025 1.583 0.010 53 2.964 0.122 4.981 0.283 0.862 0.002 3.094 0.003 1.488 0.004 54 2.699 0.070 4.736 0.195 1.024 0.007 3.589 0.020 1.778 0.008 55 2.673 0.029 5.079 0.094 0.766 0.006 3.389 0.007 1.655 0.006 56 2.314 0.019 4.518 0.113 0.884 0.001 3.495 0.022 1.773 0.018 43e1 1.977 0.025 4.520 0.056 0.806 0.003 2.330 0.008 1.082 0.008 0.325 0.158 Delta1.5(H) (3.0 mM, pH 6, 25C) # R1 error R2o error NOE error nxy error nz error Rex error 1 2.944 0.056 8.732 1.140 0.742 0.013 3.262 0.219 1.074 0.040 6.422 1.245 2 2.123 0.080 7.613 0.501 0.717 0.006 4.321 0.048 1.172 0.015 0.250 0.592 3 4 2.258 0.045 9.111 0.760 1.006 0.007 4.689 0.137 1.163 0.040 1.061 0.932 5 2.317 0.038 7.223 0.282 0.958 0.006 4.787 0.052 1.336 0.009 6 7 2.023 0.032 6.629 0.452 0.981 0.008 4.293 0.099 1.308 0.038 0.373 0.519 8 2.347 0.080 8.013 0.405 0.899 0.003 4.506 0.021 1.307 0.014 0.831 0.559 9 2.387 0.098 8.144 0.480 0.849 0.011 3.642 0.037 1.052 0.006 0.603 0.627 10 2.364 0.043 7.009 0.325 0.718 0.003 4.147 0.026 1.168 0.015 11 2.159 0.072 6.572 0.548 0.721 0.005 3.412 0.066 1.092 0.028 5.681 0.667 12 2.081 0.034 6.132 0.324 0.741 0.005 3.448 0.071 1.143 0.015 0.589 0.472 13 2.028 0.057 6.872 0.343 0.776 0.010 4.027 0.053 1.163 0.007 2.441 0.507 14 1.662 0.079 5.131 0.391 0.815 0.001 3.118 0.037 0.993 0.014 2.774 0.489 15 2.057 0.030 6.950 0.205 0.852 0.004 4.024 0.026 1.174 0.009 0.749 0.310 16 2.122 0.035 6.480 0.619 0.903 0.003 3.818 0.209 1.239 0.028 5.708 0.687 17 2.457 0.031 8.726 3.253 0.816 0.029 3.919 1.121 1.084 0.065 3.881 3.414 18 2.418 0.047 7.557 0.313 0.839 0.006 3.912 0.051 1.233 0.009 0.643 0.410 19 2.425 0.095 7.322 0.599 0.943 0.006 4.917 0.076 1.183 0.005 20 2.296 0.037 6.398 0.432 0.808 0.012 4.682 0.104 1.210 0.011 21 1.979 0.124 6.729 0.616 0.850 0.007 3.971 0.043 1.151 0.017 1.629 0.684 22 2.213 0.029 7.411 0.411 0.854 0.009 4.534 0.130 1.335 0.016 6.624 0.496 23 2.311 0.049 8.665 0.325 0.763 0.003 4.768 0.015 1.242 0.014 0.068 0.461 24 1.949 0.063 7.893 0.427 0.934 0.007 5.189 0.059 1.273 0.012 4.758 0.468 25 2.360 0.057 9.573 2.393 0.999 0.034 3.902 0.472 0.962 0.101 11.700 2.522 26 2.414 0.028 8.172 0.727 0.987 0.014 4.623 0.278 1.364 0.023 1.253 0.797 27 1.093 0.010 3.759 0.316 0.842 0.054 28 1.972 0.050 7.489 0.403 0.945 0.008 4.919 0.083 1.288 0.014 2.021 0.568 29 2.103 0.045 7.743 0.522 0.911 0.010 4.580 0.045 1.233 0.043 1.255 0.765 30 2.150 0.033 7.735 0.404 0.961 0.009 4.999 0.132 1.384 0.014 1.820 0.464 31 2.100 0.046 8.453 1.177 0.914 0.012 5.169 0.276 1.273 0.078 5.138 1.349 32 2.166 0.060 8.436 0.451 0.973 0.006 5.166 0.099 1.323 0.008 1.957 0.638 33 2.534 0.157 9.407 1.029 0.919 0.006 5.018 0.134 1.341 0.025 1.930 1.126 34 2.527 0.112 9.582 0.923 0.958 0.003 4.927 0.122 1.197 0.023 35 2.066 0.026 8.101 0.832 0.921 0.020 5.241 0.249 1.326 0.054 1.788 0.910 36 2.096 0.036 7.322 0.499 0.962 0.017 4.480 0.148 1.278 0.022 2.373 0.597 37 2.031 0.044 7.050 0.635 0.818 0.012 4.044 0.171 1.145 0.026 3.397 0.835 38 1.936 0.056 7.466 0.533 0.910 0.008 4.579 0.107 1.177 0.021 3.004 0.726 39 2.101 0.030 8.285 0.440 0.850 0.012 5.289 0.127 1.321 0.017 1.109 0.533 40 1.664 0.073 5.772 0.730 0.703 0.010 4.022 0.221 1.126 0.022 9.545 0.814 41 1.678 0.040 4.656 0.267 0.471 0.005 2.288 0.036 0.786 0.009 0.962 0.347 42 0.896 0.021 4.545 0.036 1.114 0.016 43 2.286 0.092 8.052 0.620 0.735 0.007 4.305 0.067 1.191 0.020 4.341 0.743 44 2.401 0.044 7.877 0.948 0.932 0.022 3.981 0.196 1.053 0.038 45 2.333 0.058 7.490 0.421 0.867 0.003 4.307 0.060 1.325 0.021 6.282 0.514 46 2.297 0.030 7.861 0.248 0.984 0.009 4.799 0.037 1.400 0.015 0.005 0.394 47 2.151 0.058 7.005 0.371 0.833 0.005 4.253 0.064 1.206 0.007 48 1.636 0.140 6.264 0.652 0.922 0.009 4.483 0.032 1.162 0.011 1.422 0.799 49 2.306 0.070 7.500 0.550 0.847 0.005 4.104 0.061 1.244 0.032 0.607 0.615 50 2.492 0.064 8.178 0.429 0.851 0.004 5.323 0.058 1.599 0.023 1.591 0.508 51 2.521 0.046 10.767 1.835 0.993 0.006 5.197 0.400 1.216 0.091 0.306 2.033 52 2.153 0.083 8.441 1.246 0.857 0.005 4.649 0.311 1.169 0.044 3.337 1.338 53 2.771 0.281 1.181 0.119 54 2.247 0.150 7.458 1.721 1.064 0.014 5.072 0.484 1.537 0.110 6.387 1.868 55 2.069 0.123 7.243 0.623 0.948 0.007 4.496 0.058 1.278 0.016 0.605 0.723 56 2.093 0.057 6.867 0.344 0.886 0.004 4.533 0.066 1.367 0.010 4.007 0.496 43e1 1.797 0.043 7.170 0.226 0.796 0.001 2.937 0.066 0.693 0.028 Delta1.5(L) (1.1 mM, pH 6, 25C) # R1 error R2o error NOE error nxy error nz error Rex error 1 3.442 0.064 35.300 0.4589 0.778 0.004 1.539 0.034 2 2.357 0.030 5.361 0.119 0.700 0.001 3.376 0.009 1.449 0.008 0.162 0.151 3 2.381 0.038 3.447 0.007 1.684 0.008 4 2.476 0.060 5.364 0.171 0.943 0.022 3.524 0.010 1.620 0.007 2.610 0.219 5 2.464 0.022 5.316 0.082 0.884 0.001 3.757 0.010 1.726 0.006 0.264 0.125 6 2.402 0.028 3.529 0.012 1.579 0.005 7 2.246 0.067 4.942 0.189 0.921 0.003 3.502 0.011 1.582 0.007 0.485 0.223 8 2.492 0.059 5.305 0.192 0.842 0.018 3.659 0.029 1.698 0.005 1.147 0.256 9 2.502 0.068 5.370 0.199 0.829 0.002 2.879 0.012 1.324 0.006 0.481 0.233 10 2.584 0.028 5.506 0.117 0.717 0.002 3.313 0.016 1.499 0.006 11 2.504 0.071 5.058 0.180 0.751 0.001 2.873 0.009 1.396 0.003 1.740 0.228 12 2.162 0.055 4.470 0.212 0.759 0.006 2.811 0.008 1.335 0.022 0.077 0.243 13 2.181 0.043 4.567 0.137 0.728 0.001 3.050 0.007 1.427 0.009 0.783 0.187 14 1.917 0.122 3.907 0.297 0.729 0.003 2.543 0.008 1.223 0.006 1.362 0.350 15 2.455 0.035 5.325 0.111 0.755 0.006 3.128 0.008 1.415 0.004 0.281 0.139 16 2.540 0.065 5.444 0.190 0.904 0.004 3.297 0.011 1.527 0.008 2.025 0.268 17 2.512 0.041 5.369 0.479 0.837 0.002 3.286 0.014 1.518 0.100 3.007 0.534 18 2.379 0.076 5.333 0.230 0.838 0.007 3.419 0.017 1.506 0.007 0.541 0.255 19 2.324 0.050 5.101 0.165 0.951 0.005 3.393 0.015 1.476 0.007 20 2.454 0.073 5.228 0.196 0.795 0.002 3.487 0.010 1.611 0.005 0.308 0.246 21 2.491 0.037 5.390 0.111 0.849 0.008 3.110 0.006 1.421 0.004 0.494 0.145 22 2.570 0.016 5.420 0.088 0.872 0.017 3.571 0.020 1.677 0.006 3.027 0.137 23 2.669 0.047 5.898 0.195 0.733 0.008 3.922 0.008 1.508 0.011 24 2.382 0.027 5.679 0.121 0.885 0.008 3.880 0.010 1.612 0.011 3.587 0.188 25 2.893 0.034 6.741 0.215 0.907 0.007 4.031 0.010 1.717 0.029 9.766 0.307 26 2.625 0.041 5.206 0.179 0.917 0.011 3.471 0.041 1.740 0.011 2.352 0.213 27 2.088 0.197 1.012 0.051 28 2.316 0.042 5.728 0.188 0.978 0.005 3.892 0.029 1.571 0.011 0.988 0.205 29 2.354 0.038 5.602 0.145 0.881 0.006 4.016 0.021 1.671 0.006 0.992 0.172 30 2.366 0.039 5.745 0.192 0.897 0.001 4.075 0.017 1.664 0.020 0.953 0.222 31 2.242 0.057 5.564 0.259 0.894 0.003 4.181 0.036 1.670 0.019 2.333 0.363 32 2.271 0.044 5.621 0.191 0.937 0.005 4.153 0.014 1.669 0.018 1.148 0.216 33 2.339 0.071 5.531 0.237 0.837 0.001 4.041 0.011 1.686 0.014 1.368 0.272 34 2.352 0.026 5.869 0.157 0.882 0.013 4.102 0.013 1.628 0.019 0.827 0.178 35 2.340 0.020 5.868 0.197 0.930 0.009 4.240 0.038 1.681 0.026 1.176 0.236 36 2.392 0.055 5.679 0.333 0.837 0.040 3.753 0.053 1.560 0.029 0.945 0.404 37 2.234 0.032 4.912 0.135 0.796 0.002 3.389 0.013 1.517 0.012 2.350 0.177 38 2.227 0.017 5.048 0.082 0.838 0.003 3.704 0.021 1.613 0.004 2.514 0.120 39 2.137 0.055 4.926 0.174 0.876 0.004 3.739 0.016 1.606 0.007 2.173 0.189 40 2.003 0.078 4.172 0.237 0.650 0.011 3.314 0.029 1.549 0.007 7.209 0.306 41 1.751 0.107 3.418 0.285 0.412 0.001 1.897 0.009 0.930 0.007 0.598 0.315 42 2.323 0.058 5.224 0.201 0.866 0.009 3.490 0.007 1.481 0.013 43 2.331 0.062 4.719 0.209 0.732 0.007 3.344 0.019 1.619 0.015 2.948 0.280 44 2.648 0.025 5.464 0.167 0.828 0.077 3.346 0.025 1.483 0.012 45 2.373 0.077 4.884 0.231 0.792 0.001 3.297 0.009 1.577 0.016 4.113 0.319 46 2.484 0.049 5.501 0.150 0.932 0.003 3.697 0.013 1.661 0.006 0.373 0.162 47 2.492 0.033 5.181 0.135 0.808 0.007 3.380 0.015 1.510 0.009 48 2.157 0.077 5.081 0.242 0.881 0.007 3.530 0.009 1.484 0.012 0.252 0.290 49 2.759 0.045 5.546 0.148 0.803 0.001 3.141 0.017 1.540 0.006 0.702 0.172 50 2.793 0.027 5.846 0.104 0.785 0.010 4.081 0.011 1.918 0.008 2.141 0.151 51 2.810 0.033 5.855 0.312 0.895 0.031 3.259 0.048 1.552 0.039 2.699 0.447 52 2.403 0.056 4.997 0.211 0.855 0.002 3.561 0.039 1.694 0.011 3.426 0.249 53 2.397 0.071 0.988 0.002 54 2.620 0.026 5.412 0.151 0.954 0.002 3.824 0.054 1.845 0.007 4.784 0.255 55 2.466 0.070 5.440 0.200 0.880 0.008 3.657 0.014 1.642 0.006 0.252 0.355 56 2.228 0.071 4.528 0.204 0.874 0.001 3.710 0.033 1.809 0.006 3.259 0.309 43e1 1.894 0.215 4.316 0.588 0.667 0.001 2.421 0.009 1.034 0.010 1.084 0.617