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Published November 13, 2012 | Published + Accepted Version + Supplemental Material
Journal Article Open

A microfluidic-based bubble generation platform enables analysis of physical property change in phospholipid surfactant layers by interfacial ozone reaction


The air-liquid interface filled with pulmonary surfactant is a unique feature of our lung alveoli. The mechanical properties of this interface play an important role in breathing and its malfunction induced by an environmental hazard, such as ozone, relates to various lung diseases. In order to understand the interfacial physics of the pulmonary surfactant system, we employed a microfluidic bubble generation platform with a model pulmonary surfactant composed of two major phospholipids: DPPC (1,2-dipalmitoyl-sn-phosphatidylcholine) and POPG (1-palmitoyl-2-oleoyl-sn-phosphatidylglycerol). With fluorescence imaging, we observed the ozone-induced chemical modification of the unsaturated lipid component of the lipid mixture, POPG. This chemical change due to the oxidative stress was further utilized to study the physical characteristics of the interface through the bubble formation process. The physical property change was evaluated through the oscillatory behaviour of the monolayer, as well as the bubble size and formation time. The results presented demonstrate the potential of this platform to study interfacial physics of lung surfactant system under various environmental challenges, both qualitatively and quantitatively.

Additional Information

© 2012 The Royal Society of Chemistry. The article was received on 17 Aug 2012, accepted on 16 Oct 2012 and first published on 17 Oct 2012. This work is supported by the Basic Science Research Program (Grant No. 2010-0021508, H. I. K., P. I.) through the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Science and Technology and by the National Cancer Institute Grant No. 5U54 CA119347 (J. R. H., P. I.).

Attached Files

Supplemental Material - c2lc40940b.mpg

Published - c2lc40940b.pdf

Supplemental Material - c2lc40940b_2.mpg

Supplemental Material - c2lc40940b_si.pdf

Accepted Version - nihms-478902.pdf


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August 19, 2023
August 19, 2023