The ePetri dish, an on-chip cell imaging platform based on subpixel perspective sweeping microscopy (SPSM)
We report a chip-scale lensless wide-field-of-view microscopy imaging technique, subpixel perspective sweeping microscopy, which can render microscopy images of growing or confluent cell cultures autonomously. We demonstrate that this technology can be used to build smart Petri dish platforms, termed ePetri, for cell culture experiments. This technique leverages the recent broad and cheap availability of high performance image sensor chips to provide a low-cost and automated microscopy solution. Unlike the two major classes of lensless microscopy methods, optofluidic microscopy and digital in-line holography microscopy, this new approach is fully capable of working with cell cultures or any samples in which cells may be contiguously connected. With our prototype, we demonstrate the ability to image samples of area 6 mm × 4 mm at 660-nm resolution. As a further demonstration, we showed that the method can be applied to image color stained cell culture sample and to image and track cell culture growth directly within an incubator. Finally, we showed that this method can track embryonic stem cell differentiations over the entire sensor surface. Smart Petri dish based on this technology can significantly streamline and improve cell culture experiments by cutting down on human labor and contamination risks.
Additional Information© 2011 National Academy of Sciences. Freely available online through the PNAS open access option. Edited by David A. Weitz, Harvard University, Cambridge, MA, and approved August 23, 2011 (received for review July 5, 2011). Published online before print October 3, 2011. We thank Dr. Benjamin Judkewitz for HeLa cell tracking and Mr. Samuel Yang for analyzing some of the data. We acknowledge funding support from the Coulter Foundation. Author contributions: G.Z. and C.Y. designed research; G.Z., S.A.L., and Y.A. performed research; G.Z. analyzed data; and G.Z., S.A.L., Y.A., M.B.E., and C.Y. wrote the paper.
Published - Zheng2011p16157P_Natl_Acad_Sci_Usa.pdf
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Supplemental Material - pnas.1110681108_SI.pdf