Chromosomal localization of genes encoding guanine nucleotide-binding protein subunits in mouse and human
A variety of genes have been identified that specify the synthesis of the components of guanine nucleotide-binding proteins (G proteins). Eight different guanine nucleotide-binding α-subunit proteins, two different β subunits, and one γ subunit have been described. Hybridization of cDNA clones with DNA from human-mouse somatic cell hybrids was used to assign many of these genes to human chromosomes. The retinal-specific transducin subunit genes GNAT1 and GNAT2 were on chromosomes 3 and 1; GNAI1, GNAI2, and GNAI3 were assigned to chromosomes 7, 3, and 1, respectively; GNAZ and GNAS were found on chromosomes 22 and 20. The β subunits were also assigned--GNB1 to chromosome 1 and GNB2 to chromosome 7. Restriction fragment length polymorphisms were used to map the homologues of some of these genes in the mouse. GNAT1 and GNAI2 were found to map adjacent to each other on mouse chromosome 9 and GNAT2 was mapped on chromosome 17. The mouse GNB1 gene was assigned to chromosome 19. These mapping assignments will be useful in defining the extent of the Gα gene family and may help in attempts to correlate specific genetic diseases with genes corresponding to G proteins.
Additional Information© 1988 National Academy of Sciences. Contributed by Melvin I. Simon, June 6, 1988. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact. Note Added in Proof. Kaziro and coworkers (41) isolated a human genomic clone and a rat eDNA clone designated Gxα that corresponds in sequence to the Gzα clone (10).
Published - BLApnas88.pdf