Published November 15, 1986
| Version Published
Journal Article
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Sequence of mRNA Coding for Bindin, a Species-Specific Sea Urchin Sperm Protein Required for Fertilization
Abstract
Bindin, a major protein of the sea urchin acrosome granule, mediates the species-specific adhesion and binding of sperm to egg required to effect fertilization. We report the isolation and sequence of bindin cDNA clones prepared from Strongylocentrotus purpuratus testis RNA. The bindin gene appears to be productively expressed only in males and only in testes. The protein is produced from a 51-kDa precursor, which is subsequently processed to yield the mature 24-kDa bindin protein.
Additional Information
Copyright © 1986 by the National Academy of Sciences Contributed by Eric H. Davidson, July 24, 1986 We thank Dr. V. Vacquier for the generous gift of bindin protein and bindin antibody. We also thank Dr. S. J. Horvath for providing synthetic probes and primer, and Mr. J. A. Ferrier for technical assistance. B.G. was supported in part by the California Foundation for Biomedical Research. This research was supported by Grant DCB8502191 from the National Science Foundation. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.Attached Files
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Additional details
Identifiers
- PMCID
- PMC386985
- Eprint ID
- 6642
- Resolver ID
- CaltechAUTHORS:GAOpnas86
Related works
- Describes
- http://www.pnas.org/cgi/content/abstract/83/22/8634 (URL)
Funding
- California Foundation for Biomedical Research
- NSF
- DCB8502191
Dates
- Created
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2006-12-16Created from EPrint's datestamp field
- Updated
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2019-10-02Created from EPrint's last_modified field