of 22
Neuron, Volume
90
Supplemental Information
Cholinergic Mesopontine Signals Govern Locomotion
and Reward through Dissociable Midbrain Pathways
Cheng Xiao, Jounhong Ryan Cho, Chunyi Zhou, Jennifer B. Treweek, Ken Chan, Sheri L.
McKinney, Bin Yang, and Viviana Gradinaru
2
Supplementary figures
Fig. S1, related to Fig. 1, Characterization of PPN
-to-midbrain ChAT transmission.
Whole
-
cell patch-
clamp recordings were performed in brain slices from rats with ChR2-
eYFP transduced
in PPN ChAT neurons.
(A)
A typical inward current in a midbrain neuron (V
H
= -50 mV) induced
by photo-
excitation of ChAT terminals was not blocked by atropine (1 μM), but was abolished by
the addition of MEC (10 μM).
(B)
In a typ
ical midbrain neuron, the photoexcitation-
induced c
urrent
was blocked by MEC, instead of bicuculline (10 μM).
(C)
In two typical midbrain neurons,
glutamate antagonists (50 μM APV + 20 μM CNQX) abolished inward currents in one neuron,
while reduced inward current in another neuron. Addition of MEC abolished the remaining current.
(D-E)
Neither atropine
(D)
nor bicuculline
(E)
reduced inward currents evoked by photo-
excitation
of PPN
-to-midbrain projections, but the currents were blocked by MEC.
(F)
APV + CNQX partially
(black lines) or completely (red lines
) blocked the inward currents in midbrain neurons, while MEC
blocked the remaining currents.