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Published November 1999 | Published
Journal Article Open

Single-Molecule Fluorescence Observed with Mercury Lamp Illumination


We demonstrate that it is possible to observe single fluorescent molecules using a standard fluorescence microscope with mercury lamp excitation and an inexpensive cooled charge-coupled device (CCD) camera. With this equipment, we have been able to observe single molecules of tetramethyl-rhodamine, rhodamine 6G, fluorescein isothiocyanate and green fluorescent protein. Immobilized molecules were observed both in air and in aqueous solution.

Additional Information

© 1999 Future Science Group. Received 11 February 1999; accepted 13 July 1999. The S65T, V163A, I167T, S175G GFP mutant was kindly provided by Dr. Kurt Beam, Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, CO, USA. This work was partially supported by the National Institutes of Health Grant Nos. NS-11756 and DA-9121.

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