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Published March 30, 2015 | public
Journal Article Open

High-speed label-free functional photoacoustic microscopy of mouse brain in action


We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.

Additional Information

© 2015 Macmillan Publishers Limited. Received 25 April 2014; Accepted 20 February 2015; Published online 30 March 2015. The authors appreciate J. Ballard's close reading of the manuscript. We thank C. Li, C. Chen, W. Chapman and J. Lee for discussions and technical support. This work was sponsored by US National Institutes of Health (NIH) grants DP1 EB016986 (NIH Director's Pioneer Award), R01 CA186567 (NIH Director's Transformative Research Award), 1S10 RR028864 and R01 CA159959 (to L.V.W.), and US National Science Foundation (NSF) grant CMMI-1131758 (to J.Z.). The bright-field microscopy was performed at the Alafi Neuroimaging Laboratory of the Hope Center for Neurological Disorders, Washington University School of Medicine, which is supported by the NIH Neuroscience Blueprint Center Core grant P30 NS057105. Author Contributions: J.Y., J.Z. and L.V.W. conceived of and designed the study. J.Y., J.-M.Y., K.I.M., L.W. and C.-H.H. constructed the imaging system. J.Y., T.T.W.W. and L.L. performed the experiments and analyzed the data. J.Y., L.W. and L.V.W. wrote the manuscript. L.V.W. supervised the whole study. All authors discussed the conceptual and practical implications of the methods. Competing Financial Interests: L.V.W. has a financial interest in Endra, Inc. and Microphotoacoustics, Inc., which, however, did not support this work. K.I.M. also has a financial interest in Microphotoacoustics, Inc.

Attached Files

Accepted Version - nihms677546.pdf

Supplemental Material - nmeth.3336-S1.pdf

Supplemental Material - nmeth.3336-sv1.mov

Supplemental Material - nmeth.3336-sv2.mov

Supplemental Material - nmeth.3336-sv3.mov

Supplemental Material - nmeth.3336-sv4.mov

Supplemental Material - nmeth.3336-sv5.mov


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August 20, 2023
August 20, 2023