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Published February 2012 | Published + Supplemental Material
Journal Article Open

Novel Proteomic Tools Reveal Essential Roles of SRP and Importance of Proper Membrane Protein Biogenesis

Abstract

The signal recognition particle (SRP), which mediates cotranslational protein targeting to cellular membranes, is universally conserved and essential for bacterial and mammalian cells. However, the current understanding of the role of SRP in cell physiology and pathology is still poor, and the reasons behind its essential role in cell survival remain unclear. Here, we systematically analyzed the consequences of SRP loss in E. coli using time-resolved quantitative proteomic analyses. A series of snapshots of the steady-state and newly synthesized proteome unveiled three stages of cellular responses to SRP depletion, and demonstrated essential roles of SRP in metabolism, membrane potential, and protein and energy homeostasis in both the membrane and cytoplasm. We also identified a group of periplasmic proteins, including key molecular chaperones, whose localization was impaired by the loss of SRP; this and additional results showed that SRP is crucial for protein homeostasis in the bacterial envelope. These results reveal the extensive roles that SRP plays in bacterial physiology, emphasize the importance of proper membrane protein biogenesis, and demonstrate the ability of time-resolved quantitative proteomic analysis to provide new biological insights.

Additional Information

© 2012 by The American Society for Biochemistry and Molecular Biology, Inc. Received May 27, 2011, and in revised form, September 24, 2011. Published, MCP Papers in Press, October 26, 2011. This work was supported by National Institutes of Health grant GM078024 to S.S. S.S. was supported by career awards from the Burroughs Welcome Foundation, a Beckman Young Investigator award, a Packard and Lucile award in science and engineering, and a Henry Dreyfus teacher-scholar award. S. H., R. L. J. G. and M. S. were supported by the Betty and Gordon Moore Foundation and the Beckman Institute.

Attached Files

Published - Zhang2012p17341Mol_Cell_Proteomics.pdf

Supplemental Material - mcp.M111.011585-1.doc

Supplemental Material - mcp.M111.011585-10.pdf

Supplemental Material - mcp.M111.011585-11.xlsx

Supplemental Material - mcp.M111.011585-12.xlsx

Supplemental Material - mcp.M111.011585-13.doc

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Supplemental Material - mcp.M111.011585-18.xlsx

Supplemental Material - mcp.M111.011585-19.xlsx

Supplemental Material - mcp.M111.011585-2.doc

Supplemental Material - mcp.M111.011585-20.xlsx

Supplemental Material - mcp.M111.011585-21.xlsx

Supplemental Material - mcp.M111.011585-22.xlsx

Supplemental Material - mcp.M111.011585-3.xlsx

Supplemental Material - mcp.M111.011585-4.xlsx

Supplemental Material - mcp.M111.011585-5.xlsx

Supplemental Material - mcp.M111.011585-6.xlsx

Supplemental Material - mcp.M111.011585-7.pdf

Supplemental Material - mcp.M111.011585-8.xlsx

Supplemental Material - mcp.M111.011585-9.xlsx

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Additional details

Created:
August 19, 2023
Modified:
October 24, 2023