Optical-resolution photoacoustic microscopy of angiogenesis in a transgenic mouse model
A major obstacle in studying angiogenesis is the inability to noninvasively image neovascular development in an individual animal. We applied optical-resolution photoacoustic microscopy (OR-PAM) to determine the kinetics of hypoxia-inducible factor-1 (HIF-1)-mediated angiogenesis in a transgenic mouse model. During continuous 30-day activation of HIF-1α, we used OR-PAM to monitor alterations in microvasculature in transgenic mice compared to nontransgenic mice. OR-PAM has demonstrated the potential to precisely monitor antiangiogenic therapy of human cancers, allowing for rapid determinations of therapeutic efficacy or resistance.
Additional Information© 2010 Society of Photo-Optical Instrumentation Engineers (SPIE). The authors thank Rebecca Sohn for mouse husbandry, Rick Bruick for the gift of the HIF-1α^(PPN) plasmid for construction of the TRE-HIF-1α^(PPN) transgenic mice, Adam Glick for the gift of the K5-rtTa transgenic mice, and ImClone for the generous gift of the DC101, and MF1 antibodies, Fulu Liu, Alyssa Gregory, Kyle Eash and Dan Link for help with bone marrow transplantation and advice on AMD3100 experiments. This work was supported by NCI R01-CA90722, National Institutes of Health Grants R01 EB000712, R01 NS46214, R01 EB008085, and U54 CA136398. L.V.W. has financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
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