CaltechAUTHORS
Published January 11, 2012 | Version Supplemental Material
Journal Article Open

Bacterial Isolation by Lectin-Modified Microengines

Creators

  • 1. ROR icon University of California, San Diego
  • 2. ROR icon Complutense University of Madrid

Abstract

New template-based self-propelled gold/nickel/polyaniline/platinum (Au/Ni/PANI/Pt) microtubular engines, functionalized with the Concanavalin A (ConA) lectin bioreceptor, are shown to be extremely useful for the rapid, real-time isolation of Escherichia coli (E. coli) bacteria from fuel-enhanced environmental, food, and clinical samples. These multifunctional microtube engines combine the selective capture of E. coli with the uptake of polymeric drug-carrier particles to provide an attractive motion-based theranostics strategy. Triggered release of the captured bacteria is demonstrated by movement through a low-pH glycine-based dissociation solution. The smaller size of the new polymer-metal microengines offers convenient, direct, and label-free optical visualization of the captured bacteria and discrimination against nontarget cells.

Additional Information

© 2011 American Chemical Society. Received 21 October 2011. Published online 5 December 2011. Published in print 11 January 2012. This work was supported by the National Science Foundation (Award Number CBET 0853375), the National Institutes of Health (Award U01 AI075565), NATO Science for Peace and Security Program (SfP 983807), and the Laboratory Directed Research and Development program at Sandia National Laboratories. S.C. and J.O. acknowledge the support from Programa Becas Complutense del Amo (2010-2011) and a Beatriu de Pinós postdoctoral fellowship (Government of Catalonia), respectively. M.G. thanks the University Autónoma of Barcelona (UAB) and the Spanish MICINN. The authors would like to acknowledge also B. Chuluun and C. Halford for their technical assistance.

Attached Files

Supplemental Material - nl203717q_si_001.pdf

Supplemental Material - nl203717q_si_002.mpg

Supplemental Material - nl203717q_si_003.mpg

Supplemental Material - nl203717q_si_004.mpg

Supplemental Material - nl203717q_si_005.mpg

Supplemental Material - nl203717q_si_006.mpg

Supplemental Material - nl203717q_si_007.mpg

Supplemental Material - nl203717q_si_008.mpg

Supplemental Material - nl203717q_si_009.mpg

Supplemental Material - nl203717q_si_010.mpg

Files

nl203717q_si_001.pdf

Files (31.6 MB)

Name Size Download all
md5:c31e0f936ce9d768235ec15d184926c5
80.4 kB Preview Download
md5:5d93326206cde46c985d16dbfe855de0
5.6 MB Download
md5:9f5afa31084e59fef747de691bc54784
5.0 MB Download
md5:5c5c2d0ecb94060384fc690f3e69e074
5.3 MB Download
md5:ae6d169dc0b83e4b8265955132a1520e
3.2 MB Download
md5:3f53206ab588d68a45fa072bf5eadeb3
3.8 MB Download
md5:af142a453f31c0c6b990f316e92068c6
1.1 MB Download
md5:d40361a34942a1f621aaefd979e1a178
1.8 MB Download
md5:43838b939fde75ad8237a57be32fd144
2.6 MB Download
md5:baaca19fb4461a04d40f422e1466fb90
3.2 MB Download

Additional details

Identifiers

Eprint ID
84543
DOI
10.1021/nl203717q
Resolver ID
CaltechAUTHORS:20180126-104905077

Related works

Describes
10.1021/nl203717q (DOI)

Funding

NSF
CBET-0853375
NIH
U01 AI075565
North Atlantic Treaty Organization (NATO)
SfP 983807
Sandia National Laboratories
Programa Becas Complutense del Amo (2010-2011)
Beatriu de Pinós (Catalonia)
University Autónoma of Barcelona
Ministerio de Ciencia e Innovación (MCINN)

Dates

Created
2018-01-31
Created from EPrint's datestamp field
Updated
2021-11-15
Created from EPrint's last_modified field