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Published March 2, 2009 | Accepted Version
Journal Article Open

Fluorescence Detection of a Protein-Bound 2Fe2S Cluster


A fluorescent biosensor is described for 2Fe2S clusters that is composed of green fluorescent protein (GFP) fused to glutaredoxin 2 (Grx2), as illustrated here. 2Fe2S detection is based on the reduction of GFP fluorescence upon the 2Fe2S-induced dimerization of GFP-Grx2. This assay is sufficiently sensitive to detect submicromolar changes in 2Fe2S levels, thus making it suitable for high-throughput measurements of metallocluster degradation and synthesis reactions.

Additional Information

© 2009 Wiley. Issue online: 24 Feb 2009 Version of Record online: 30 Jan 2009 Manuscript Received: 12 Nov 2008 This work was supported by the Robert A. Welch Foundation (C-1614; to J.J.S.), Arnold and Mabel Beckman Foundation (to C.D.S.), and a National Institutes of Health Biotechnology fellowship (5F32M078901; to K.G.H.).

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Accepted Version - nihms149605.pdf


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