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Published February 2018 | public
Journal Article

Correcting the limited view in optical-resolution photoacoustic microscopy


Optical-resolution photoacoustic microscopy (OR-PAM) has proven useful for anatomical and functional imaging with high spatial resolutions. However, the coherent signal generation and the desired reflection-mode detection in OR-PAM can result in a limited detectability of features aligned with the acoustic axis (i.e., vertical structures). Here, we investigated the limited-view phenomenon in OR-PAM by simulating the generation and propagation of the acoustic pressure waves and determined the key optical parameters affecting the visibility of vertical structures. Proof-of-concept numerical experiments were performed with different illumination angles, optical foci, and numerical apertures (NA) of the objective lens. The results collectively show that an NA of 0.3 can readily improve the visibility of vertical structures in a typical reflection-mode OR-PAM system. This conclusion was confirmed by numerical simulations on the cortical blood vessels in a mouse brain, and by experiments in a suture-cross phantom and in a mouse brain in vivo.

Additional Information

© 2017 Wiley. Accepted manuscript online: 3 October 2017; Manuscript Accepted: 27 September 2017; Manuscript Revised: 14 September 2017; Manuscript Received: 27 July 2017. Funded by: Duke MEDx.

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August 21, 2023
October 17, 2023