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Published April 1950 | public
Journal Article Open

A colorimetric method for the determination of chymotrypsin activity


The L isomers of esters, amides, and hydrazides of the general formula RCONHCHR1COR2 where R = CH3-, C6H5-, etc., R1 = C6H6CH2-, p-HO-C6H4CH2-, etc., and R2 = -OCH3, -NH2, or -NHNH2, are known to be hydrolyzed by chymotrypsin (l-6). It has now been found that the corresponding hydroxamides, RCONHCHR1CONHOH, where R1 = C6H5CH2-, are also hydrolyzed by this enzyme (Table I). Aside from the obvious usefulness of the above hydroxamides in the further definition of chymotrypsin activity we wish to point out that these latter substrates provide the basis for a simple, sensitive, and rapid calorimetric method for the determination of chymotrypsin activity which can be extended to a number of other proteolytic enzymes.

Additional Information

Copyright © 1950 by the American Society of Biological Chemists (Received for publication, October 31, 1949) Supported in part by a grant from Eli Lilly and Company. Contribution No. 1348 from the Gates and Crellin Laboratories of Chemistry, California Institution of Technology, Pasadena


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