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Published February 1990 | public
Journal Article

A v-myc-Immortalized Sympathoadrenal Progenitor Cell Line in Which Neuronal Differentiation Is Initiated by FGF but Not NGF


Sympathetic neurons differentiate from a developmentally restricted progenitor cell in the neural crest-derived sympathoadrenal lineage. We have isolated these progenitors by fluorescence-activated cell sorting and immortalized them using a v-myc-containing retrovirus. The complement of antigenic markers expressed by these lines suggests that they have retained many of the properties of their normal counterparts.These lines initiate neuronal differentiation in response to basic FGF, but not to NGF, and do not contain NGF receptor mRNA. In NGF plus FGF, however, a small percentage of the cells differentiate to NGF-dependent postmitotic neurons. Furthermore, an induction of NGF receptor mRNA can be observed in response to FGF Thus, the development of sympathetic neurons may involve a relay, in which FGF both initiates differentiation and induces the NGF receptor, which in turn controls further maturation and survival.

Additional Information

© 1990 by Cell Press. Received 14 September 1989, Revised 15 November 1989. We are indebted to Drs. Connie Cepko, Luis Parada, Ron McKay, and Owen Witte for providing virus stocks and advice on retroviral immortalization. We thank Dr. Sigrun Korsching for her gift of anti-NGF antibody and Mr. Gary Holt for his contributions to the immunocytochemical characterization of MAH cells. We are grateful to Drs. Moses Chao and Eric Shooter for providing NGF receptor cDNA probes. We acknowledge the excellent technical assistance of Ms. liching Lo and Mr. Steven Padilla, Ms. Rochelle Diamond for performing cell sorting, and the help of Ms. Helen Walsh in the preparation of the manuscript. We thank Barbara Wold and Allison Doupe for their critical comments and Paul Patterson and Josette Carnahan for sharing their unpublished data. S. B. is supported by Damon Runyan-Walter Winchell Cancer Research Fund fellowship no. DRG-956. This work was supported in its initial stages by NIH grant NS23476, and subsequently by a PEW faculty fellowship in Neurosciences to D. J. A. D. J. A. is an assistant investigator of the Howard Hughes Medical Institute.

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