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Published September 1981 | Published
Journal Article Open

Heat-shock proteins of Drosophila are associated with nuclease-resistant, high-salt-resistant nuclear structures


Proteins produced in cultured Drosophila cells during the heat-shock response (HSPs) were recently shown by autoradiography to be confined in large measure to the cell nucleus. We report here that nuclear HSPs are not associated with nucleosomes solubilizes by treatment with staphylococcal nuclease at low ionic strength nor are HSPs released by extraction with high salt, which solubilized most of the remaining histones and DNA. Possible functions of nuclear HSPs are discussed.

Additional Information

© 1981 The Rockefeller University Press. Received for publication 12 February 1981, and in revised form 4 June 1981. We gratefully acknowledge repeated gifts of Drosophila cells from the laboratory of Mary Lou Pardue and thank her and Karen Traverse for helpful discussions. We also thank Olof Sundin and James Barsoum for a critical reading of the manuscript and Nancy Fritz for secretarial assistance. This work was supported by a grant to A. Varshavsky from the National Institutes of Health (NIH) (GM26086). L. Levinger is supported by an NIH postdoctoral fellowship (AI5932).

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