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Published September 14, 2005 | public
Journal Article Open

Bioconjugates for Tunable Peptide Fragmentation: Free Radical Initiated Peptide Sequencing (FRIPS)


The free radical initiator Vazo 68 is coupled to a peptide and electrosprayed into an ion trap mass spectrometer. On collisional activation, the Vazo 68−peptide conjugate generates a free radical, which can be collisionally activated to cleave the peptide backbone. Mostly z-type fragments are formed, as in CAD of other radical peptides and ECD fragmentation. We present data for the Angiotensin II−Vazo 68 conjugate and discuss possible sites of H atom abstraction from the peptide. This experimental methodology for generating peptide fragments is a useful step toward the development of a completely gas-phase approach to protein sequencing.

Additional Information

© 2005 American Chemical Society. Received March 30, 2005. Publication Date (Web): August 17, 2005. This material is based upon work supported by the NSF under Grant No. CHE-0416381, and the Beckman Institute at Caltech. We thank Gary Kruppa of Bruker BioSpin for assistance with FT-MS corroboratory data.

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Supplemental Material - ja052042zsi20050802_060721.pdf


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August 19, 2023
August 19, 2023