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Published May 17, 2013 | Version Accepted Version + Supplemental Material
Journal Article Open

High-Throughput Screening for Terpene-Synthase-Cyclization Activity and Directed Evolution of a Terpene Synthase

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Abstract

The development of high-throughput assays can be extremely challenging, yet is essential for many applications in drug discovery and enzyme engineering.

Additional Information

© 2013 Wiley-VCH Verlag GmbH & Co. Received: February 15, 2013; Published online: March 26, 2013. We are grateful to David Cane, Scott Virgil, Robert M. Coates, and David Christianson for their pioneering studies, invaluable advice, encouragement, and materials. We thank Indira Wu, Chris Farwell, and Jack Zhang for assistance. R.L. acknowledges the support of NIH fellowship F32GM095061. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. K.S.R. thanks the Deutscher Akademischer Austauschdienst (DAAD) for a postdoctoral fellowship. K.Z.K. and R.Z.K. acknowledge the support of Summer Undergraduate Research Fellowships from the California Institute of Technology. T.H. was funded by the FWF grant number: J3327-B21.

Attached Files

Accepted Version - nihms523186.pdf

Supplemental Material - Supporting_Information.pdf

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Additional details

Additional titles

Alternative title
High-throughput screening and directed evolution of terpene synthase-catalyzed cylization

Identifiers

PMCID
PMC3828198
Eprint ID
39162
Resolver ID
CaltechAUTHORS:20130701-105908055

Funding

NIH
F32GM095061
Deutscher Akademischer Austauschdienst (DAAD)
Caltech Summer Undergraduate Research Fellowship (SURF)
FWF Der Wissenschaftsfonds
J3327-B21

Dates

Created
2013-07-01
Created from EPrint's datestamp field
Updated
2021-11-09
Created from EPrint's last_modified field