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Published April 1990 | public
Journal Article

Analysis of SCG10 Gene Expression in Transgenic Mice Reveals That Neural Specificity Is Achieved through Selective Derepression


SCG10 is a neural-specific, growth-associated protein that is broadly expressed in the embryonic central and peripheral nervous systems. Transgenic mice harboring a chimeric gene containing 4 kb of SCG10 5′ flanking DNA fused to the bacterial CAT gene exhibit expression in brain but not in nonneuronal tissues. A low level of expression is detected in adrenal gland as well, consistent with the behavior of endogenous SCG10. Such a transgene is also activated at the same relative stage of embryonic development as its endogenous counterpart. Deletion of the 5′-most 3.7 kb of SCG10 sequence yields deregulated expression of the transgene in numerous nonneuronal tissues, although expression remains highest in brain. In contrast to other tissue-specific genes, therefore, the specificity of SCG10 expression appears to be achieved predominantly through selective repression in nonneuronal tissues.

Additional Information

© 1990 by Cell Press. Received 26 December 1989, Revised 2 February 1990. We are grateful to Jesse Dausman for instruction in the transgenic mouse technique, to Jane Johnson for helpful suggestions, and to Carol Readhead for managing the Transgenic Animal Facility at Caltech. We thank Steven Padilla for technical assistance and Barbara Wold and Chris Schoenherr for constructive comments on the manuscript. This work was supported by a Markey Internal Grant in Developmental Biology and by Searle Scholars and National Science Foundation Presidential Young Investigator awards to D. J. A. C. W.W. was supported by a National Institutes of Health postdoctoral fellowship. D. j. A. is an Assistant Investigator, and C. W.W. and N. M. are Associates, of the Howard Hughes Medical Institute.

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