Effect of the roots on the production of auxin by the coleoptile

Since it had been shown by Skoog (1936, 1937) that removal of the seed (endosperm and scutellum) of Avena seedlings greatly reduces the production of auxin in the coleoptile tip, the question arose whether removal of the root system would have any effect upon the auxin relations of the plant. This was the purpose of the present investigation.