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Published September 2008 | Published
Journal Article Open

Fast 3-D dark-field reflection-mode photoacoustic microscopy in vivo with a 30-MHz ultrasound linear array


We present an in vivo dark-field reflection-mode photoacoustic microscopy system that performs cross-sectional (B-scan) imaging at 50Hz with real-time beamforming and 3-D imaging consisting of 166 B-scan frames at 1Hz with postbeamforming. To our knowledge, this speed is currently the fastest in photoacoustic imaging. A custom-designed light delivery system is integrated with a 30-MHz ultrasound linear array to realize dark-field reflection-mode imaging. Linear mechanical scanning of the array produces 3-D images. The system has axial, lateral, and elevational resolutions of 25, 70, and 200μm, respectively, and can image 3mm deep in scattering biological tissues. Volumetric images of subcutaneous vasculature in rats are demonstrated in vivo. Fast 3-D photoacoustic microscopy is anticipated to facilitate applications of photoacoustic imaging in biomedical studies that involve dynamics and clinical procedures that demand immediate diagnosis.

Additional Information

© 2008 SPIE. Paper 08069R received Feb. 28, 2008; revised manuscript received May 22, 2008; accepted for publication May 23, 2008; published online Sep. 11, 2008. This work was sponsored, in part, by Grants No. R01 EB000712 and No. R01 NS46214 from the National Institutes of Health.

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Published - SongL_2008_J_Biomed_Opt_13_054028.pdf


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