Cell, Volume
184
Supplemental Information
A circuit logic for sexually shared
and dimorphic aggressive behaviors in
Drosophila
Hui Chiu, Eric D. Hoopfer, Maeve L. Coughlan, and David J. Anderson
Table&S1.&Genotypes&of&flies&used&in&each&experiment.&Related&to&all&figures.
Figure&1
Abbreviation
Genotypes
1B#C
Canton#S*
Figure&2
Abbreviation
Genotypes
2B
Eb5#Gal4(attp40)*x*20xUAS#CshChrimson#mVenus(attp2)
Eb5>CsChR
Eb5#Gal4(attp40)*x*20xUAS#CshChrimson#mVenus(attp2)
Eb5>GFP
Eb5#Gal4(attp40)*x*pJFRC82#20xUAS#IVS#Syn21#GFP#p10(attp2)
2Di#ii*&*2Ei
Eb5#Gal4(attp2)*x*UAS#C3PA#GFP*(III)
2Diii#v
CAP*driver
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
2Eii
pC1*driver
NP2631N*dsx#Flp*x*20xUAS#FRT#myrTopHat2#FRT#Chrimson::tdT3.1(vk5)
2Eiii
MAP*driver
Eb5#AD(vk27),*22F05#DBD(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
Figure&3
Abbreviation
Genotypes
CAP>CsChR*(tester)
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
CAP>GFP*(control)
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*pJFRC82#20xUAS#IVS#Syn21#GFP#p10(attp2)
MAP>CsChR*(tester)
Eb5#AD(vk27),*22F05#DBD(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
MAP>GFP*(control)
Eb5#AD(vk27),*22F05#DBD(attp2)*x*pJFRC82#20xUAS#IVS#Syn21#GFP#p10(attp2)
CAP>Kir2.1*(tester)
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*pJFRC49#10xUAS#eGFP::Kir2.1(attp2)
CAP>GFP*(control)
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*pJFRC81#10xUAS#IVS#Syn21#GFP#p10*(attp2)
Figure&4
Abbreviation
Genotypes
CAP>CsChR*(tester)
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
CAP>GFP*(control)
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*pJFRC82#20xUAS#IVS#Syn21#GFP#p10(attp2)
4B
Eb5iLexA(attp18)N*20xUAS#IVS#Syn21#Chrimson::tdT3.1(su(Hw)attp5)N*13xLexAop#IVS#jGCaMP7b(vk5)*x*Eb5#Gal4(attp40)N*22F05#Gal80(attp2)
Figure&5
Abbreviation
Genotypes
CAP>CsChR*(tester)
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
CAP>GFP*(control)
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*pJFRC82#20xUAS#IVS#Syn21#GFP#p10(attp2)
MAP>CsChR*(tester)
Eb5#AD(vk27),*22F05#DBD(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
MAP>GFP*(control)
Eb5#AD(vk27),*22F05#DBD(attp2)*x*pJFRC82#20xUAS#IVS#Syn21#GFP#p10(attp2)
MAP>Kir2.1*(tester)
Eb5#AD(vk27),*22F05#DBD(attp2)*x*pJFRC49#10xUAS#eGFP::Kir2.1(attp2)
MAP>GFP(control)
Eb5#AD(vk27),*22F05#DBD(attp2)*x*pJFRC81#10xUAS#IVS#Syn21#GFP#p10*(attp2)
CAP+MAP>ChR+MAP>Kir2.1
Eb5#iLexA(attp18)N+N*Eb5#AD(vk27)N*22F05#DBD(attp2)**x*13xLexAop2#IVS#Syn21#Chrimson#tdTomato#3.1(su(Hw)attp5)N*pJFRC49#10xUAS#eGFP::Kir2.1(attp2)
CAP+MAP>ChR+Empty>Kir2.1
Eb5#iLexA(attp18)NBDP#AD(attp40)N*BDP#DBD(attp2)**x*13xLexAop2#IVS#Syn21#Chrimson#tdTomato#3.1(su(Hw)attp5)N*pJFRC49#10xUAS#eGFP::Kir2.1(attp2)
Figure&6
Abbreviation
Genotypes
6Ai
MAP*neurons
Eb5#AD(vk27),*22F05#DBD(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
6Aii
CAP(Chrimson)##>MAP(jG7b)
Eb5iLexA(attp18)N*20xUAS#IVS#Syn21#Chrimson::tdT3.1(su(Hw)attp5)N*13xLexAop#IVS#jGCaMP7b(vk5)*x*Eb5#Gal4(attp40)N*22F05#Gal80(attp2)
6Bi
fpC1*neurons
26E01#AD(attp40)N*dsx#DBD*x*20xUAS#CsChrimson#mVenus(attp2)
6Bii
CAP(Chrimson)##>fpC1(jG7b)
Eb5iLexA(attp18)N*13xLexAop2#Chrimson::tdT3.1*(su(Hw)attp5)N*20xUAS#jGCaMP7b*(vk5)*x*26E01#AD(attp40)N*dsx#DBD
fpC1>Chrimson
dsxFlp*x*26E01#Gal4(attp2),*20xUAS#FRT#myrTopHat2#FRT#Chrimson::tdT3.1(vk5)
fpC1>STOP
dsxFlp*x*BDPGal4U(attp2),*20xUAS#FRT#myrTopHat2#FRT#Chrimson::tdT3.1(vk5)
fpC1>Kir2.1
26E01#AD(attp40)N*dsx#DBD*x*pJFRC49#10xUAS#eGFP#Kir2.1(attp2)
fpC1>GFP
26E01#AD(attp40)N*dsx#DBD*x*pJFRC81#10xUAS#IVS#Syn21#GFP#p10*(attp2)
CAP>Chrimson+*fpC1>Kir2.1
Eb5iLexA(attp18)N*13xLexAop2#IVS#Syn21#Chrimson::tdT3.1(su(Hw)attp5)N*pJFRC49#10xUAS#eGFP#Kir2.1(attp2)*x*26E01#AD(attp40)N*dsx#DBD
CAP>Chrimson+*Empty>Kir2.1
Eb5iLexA(attp18)N*13xLexAop2#IVS#Syn21#Chrimson::tdT3.1(su(Hw)attp5)N*pJFRC49#10xUAS#eGFP#Kir2.1(attp2)*x*BDP#AD(attp40)N*BDP#DBD(attp2)
Figure&7
Abbreviation
Genotypes
7Ai
CAP##>MAP
Eb5iLexA(attp18)N*20xUAS#IVS#Syn21#Chrimson::tdT3.1(su(Hw)attp5)N*13xLexAop#IVS#jGCaMP7b(vk5)*x*Eb5#Gal4(attp40)N*22F05#Gal80(attp2)
7Aii
CAP##>fpC1
Eb5iLexA(attp18)N*13xLexAop2#Chrimson::tdT3.1*(su(Hw)attp5)N*20xUAS#jGCaMP7b*(vk5)*x*26E01#AD(attp40)N*dsx#DBD
CAP>CsChrimson
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
CAP>GFP
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*pJFRC82#20xUAS#IVS#Syn21#GFP#p10(attp2)
Figure&S1
Abbreviation
Genotypes
S1A*&*S1C
Wild#type*approach
Canton#S
S1B
CAP#induced*approach
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
S1D
Wild#type*approach
Canton#S
Figure&S2
Abbreviation
Genotypes
R60G08>CsChrimson
R60G08#Gal4(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
BDP>CsChrimson
BDPG4U(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
S2B
R60G08#Gal4(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
R60G08+FlpON
R60G08#Gal4(attp2)*x*20xUAS#FRT#myrTopHat2#FRT#Chrimson::tdT3.1(vk5)
R60G08+otdFlp+FlpON
R60G08#Gal4(attp2)*x*otd#Flp(attp40)N*20xUAS#FRT#myrTopHat2#FRT#Chrimson::tdT3.1(vk5)
R60G08+FlpOFF
R60G08#Gal4(attp2)*x*20xUAS#FRT#Chrimson::tdT3.1#FRT#myrTopHat2(vk5)
R60G08+otdFlp+FlpOFF
R60G08#Gal4(attp2)*x*otd#Flp(attp40)N*20xUAS#FRT#Chrimson::tdT3.1#FRT#myrTopHat2(vk5)
Figure&S3
Abbreviation
Genotypes
Eb2#Gal4(attp2)
Eb2#Gal4(attp40)*x*20xUAS#CshChrimson#mVenus(attp2)
Eb3#Gal4(attp2)
Eb3#Gal4(attp40)*x*20xUAS#CshChrimson#mVenus(attp2)
Eb4#Gal4(attp2)
Eb4#Gal4(attp40)*x*20xUAS#CshChrimson#mVenus(attp2)
Eb5#Gal4(attp2)
Eb5#Gal4(attp40)*x*20xUAS#CshChrimson#mVenus(attp2)
60G08*neurons
R60G08#Gal4(attp2)*x*20xUAS#IVS#Syn21#Chrimson#tdTomato#3.1*(su(Hw)attp5)
Eb5*neurons
Eb5#Gal4(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
60G08+/Eb5#*neurons
R60G08#Gal4(attp2)*x*20xUAS#IVS#Syn21#Chrimson#tdTomato#3.1*(su(Hw)attp5)N*Eb5#Gal80(attp2)
S3Ci#ii
Eb5*vs*dsx#Gal4
Eb5iLexA(attp18)N*20xUAS#Chrimson::tdT3.1(su(Hw)att5)N*13xLexAop#jGCaMP7b(vk5)*x*dsx#Gal4
S3Ciii
Eb5*vs*FruM
Eb5iLexA(attp18)*x*13xLexAop2#CsChrimson::tdT3.1*(vk5)*
S3Ei#ii
CAP*vs*Eb5
Eb5iLexA(attp18)N*10xUAS#nls::tdTomato(vk22)N*13xLexAop2#nls::GFP(vk40)*x*Eb5#Gal4(attp40)N*22F05#Gal80(attp2)
S3Eiii#iv
MAN*vs*Eb5
Eb5iLexA(attp18)N*10xUAS#nls::tdTomato(vk22)N*13xLexAop2#nls::GFP(vk40)*x*Eb5#AD(vk27)N*22F05#DBD(attp2)
Figure&S4
Abbreviation
Genotypes
S4Ai*
MAP*
Eb5#AD(vk27),*22F05#DBD(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
MAP>CsChR
Eb5#AD(vk27),*22F05#DBD(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
MAP>GFP(control)
Eb5#AD(vk27),*22F05#DBD(attp2)*x*pJFRC82#20xUAS#IVS#Syn21#GFP#p10(attp2)
Eb5#Gal4*
Eb5#Gal4(attp40)*x*20xUAS#CsChrimson#mVenus(attp2)
CAP
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
MAP
Eb5#AD(vk27),*22F05#DBD(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
fpC1
26E01#AD(attp40)N*dsx#DBD*x*20xUAS#CsChrimson#mVenus(attp2)
S4D
NP2631N*Eb5#AD(vk27),*22F05#LexADBD(attp2)*x*10xUAS#nls::tdTomato(vk22)N*13xLexAop2#nls::GFP(vk40)
S4Ei
fpC1>CsChrimson
26E01#AD(attp40)N*dsx#DBD*x*20xUAS#CsChrimson#mVenus(attp2)
fpC1+FlpON
26E01#AD(attp40)N*dsx#DBD*x*20xUAS#FRT#myrTopHat2#FRT#Chrimson::tdT3.1(vk5)
fpC1+otdFlp+FlpON
26E01#AD(attp40)N*dsx#DBD*x*otd#Flp(attp40)N*20xUAS#FRT#myrTopHat2#FRT#Chrimson::tdT3.1(vk5)
fpC1+FlpOFF
26E01#AD(attp40)N*dsx#DBD*x*20xUAS#FRT#Chrimson::tdT3.1#FRT#myrTopHat2(vk5)
fpC1+otdFlp+FlpOFF
26E01#AD(attp40)N*dsx#DBD*x*otd#Flp(attp40)N*20xUAS#FRT#Chrimson::tdT3.1#FRT#myrTopHat2(vk5)
MAP>GCaMP7b
Eb5iLexA(attp18)N*20xUAS#IVS#Syn21#Chrimson::tdT3.1(su(Hw)attp5)N*13xLexAop#IVS#jGCaMP7b(vk5)*x*Eb5#Gal4(attp40)N*22F05#Gal80(attp2)
fpC1>GCaMP7b
Eb5iLexA(attp18)N*13xLexAop2#Chrimson::tdT3.1*(su(Hw)attp5)N*20xUAS#jGCaMP7b*(vk5)*x*26E01#AD(attp40)N*dsx#DBD
Figure&S5
Abbreviation
Genotypes
S5A
Eb5>CsChR*(tester)
Eb5#Gal4(attp40)*x*20xUAS#CsChrimson#mVenus(attp2)
CAP>CsChR*(tester)
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
CAP>GFP*(control)
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*pJFRC82#20xUAS#IVS#Syn21#GFP#p10(attp2)
CAP>Kir2.1*(tester)
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*pJFRC49#10xUAS#eGFP::Kir2.1(attp2)
CAP>GFP*(control)
Eb5#Gal4(attp40)N*22F05#Gal80(attp2)*x*pJFRC81#10xUAS#IVS#Syn21#GFP#p10*(attp2)
MAP>Kir2.1*(tester)
Eb5#AD(vk27),*22F05#DBD(attp2)*x*pJFRC49#10xUAS#eGFP::Kir2.1(attp2)
MAP>GFP(control)
Eb5#AD(vk27),*22F05#DBD(attp2)*x*pJFRC81#10xUAS#IVS#Syn21#GFP#p10*(attp2)
fpC1>Kir2.1(tester)
26E01#AD(attp40)N*dsx#DBD*x*pJFRC49#10xUAS#eGFP#Kir2.1(attp2)
fpC1>GFP*(control)
26E01#AD(attp40)N*dsx#DBD*x*pJFRC81#10xUAS#IVS#Syn21#GFP#p10*(attp2)
MAP>CsChR
Eb5#AD(vk27),*22F05#DBD(attp2)*x*20xUAS#CsChrimson#mVenus(attp2)
fpC1>CsChR
26E01#AD(attp40)N*dsx#DBD*x*20xUAS#CsChrimson#mVenus(attp2)
Figure&S6
Abbreviation
Genotypes
S6A
Eb5iLexA(attp18)N*20xUAS#IVS#Syn21#Chrimson::tdT3.1(su(Hw)attp5)N*13xLexAop#IVS#jGCaMP7b(vk5)*x*Eb5#Gal4(attp40)N*22F05#Gal80(attp2)
S6B
Eb5#AD(vk27),*22F05#DBD(attp2)*x*20xUAS#IVS#Syn21#Chrimson::tdT3.1(su(Hw)attp5)N*20xUAS#IVS#jGCaMP7b(vk5)*
S6C
26E01#AD(attp40)N*dsx#DBD*x*20xUAS#IVS#Syn21#Chrimson::tdT3.1(su(Hw)attp5)N*20xUAS#IVS#jGCaMP7b(vk5)*
Figure&S7
Abbreviation
Genotypes
Eb5>Chrimson*+*tk>Kir2.1
tk#Gal4N*Eb5iLexA(attp40)*x*13xLexAop2#Chrimson::tdT3.1(su(Hw)attp5)N*10xUAS#eGFP#Kir2.1(attp2)
Eb5>Chrimson*+*Empty>Kir2.1
Eb5iLexA(attp40)*x*13xLexAop2#Chrimson::tdT3.1(su(Hw)attp5)N*10xUAS#eGFP#Kir2.1(attp2)
tk>Chrimson*+*Eb5*>*Kir2.1
tk#Gal4N*Eb5iLexA(attp40)*x*13xLexAop2#eGFP#Kir2.1(attp40)N*20xUAS#Chrimson::tdT3.1(vk5)
tk>Chrimson*+*Empty*>*Kir2.1
tk#Gal4*x*13xLexAop2#eGFP#Kir2.1(attp40)N*20xUAS#Chrimson::tdT3.1(vk5)
S4B
S4Eii
S4F
S7A
S7B
S5B
S5D
S5Ci#ii
S5Ciii
S5Ciii
2C
5A*&*5D
5C
4A
7B*&*7C
6C
6D
6E
5B
S4Aii
3Ai,*3Aiii,*&*3B
3Aii
3C
S3B
S2A
S2C
S3A
Table&S2.&Behavioral&classifier&performance.&Related&to&Figure&1.
Precision&(%)
!=!
TP/(TP+FP)
Recall&(%)&&&
=!
TP/(TP+FN)
True&Positive&
(TP)
True&Negative&&
(TN)
False&Positive&
(FP)!
False&Negative&
(FN)
Approach
92.2
89.6
879!(92.2%)
1431!(93.3%)
74!(7.8%)
102!(6.7%)
Lunging
94.4
87.1
304!(94.4%)
1388(96.9%)
18(5.6%)
45(3.1%)
Headbutting
78.4
89.9
1344!(78.4%)
1147(88.4%)
371(21.6%)
151!(11.6%)
Precision&(TP/(TP+FP)):&
percentage!of!the!detected!behavioral!frames!that!were!true!positives
Recall&(TP/(TP+FN)):&
percentage!of!the!true!behavioral!frames!that!were!detected
True&Positive&(TP):
!percentage!of!the!frames!in!which!animals!displayed!certain!behavior!and!were!correctly!detected!by!the!classifer
True&Negative&(TN):
!percentage!of!the!frames!in!which!animals!did!not!display!certain!behavior!and!were!correctly!distingished!by!the!classifer
False&Positive&(FP):&
percentage!of!the!frames!in!which!animals!did!not!display!certain!behavior!but!were!incorrectly!detected!by!the!classifier
False&Negative&(FN)
:!percentage!of!the!frames!in!which!animals!displayed!certain!behavior!but!were!not!detected!by!the!classifier
No.&of&training&frames&
Performance
Classifiers
Table&S3.&Statistical¶meters&of&each&experiment.&Related&to&all&figures.
Figure&1
Comparisons&&(Group&1&(G1)&vs&Group&2&(G2))
N&number
Methods
p
&value
U&value
W&value
z&value
signedrank
tstat
df
sd
Group&housed+(GH)+males+vs+GH+females
8+pairs+vs+8+pairs
0.006993
8
44
Single&housed+(SH)+males+vs+SH+females
8+pairs+vs+8+pairs
2.95E&03
5
95
GH+males+vs+SH+males
8+pairs+vs+8+pairs
1.55E&04
0
36
GH+females+vs+SH+females
8+pairs+vs+8+pairs
3.11E&04
1
37
Group&housed+(GH)+males+vs+GH+females
8+pairs+vs+8+pairs
2
32
68
Single&housed+(SH)+males+vs+SH+females
8+pairs+vs+8+pairs
1.55E&04
0
100
GH+males+vs+SH+males
8+pairs+vs+8+pairs
1.55E&04
0
36
GH+females+vs+SH+females
8+pairs+vs+8+pairs
1
28
64
Group&housed+(GH)+males+vs+GH+females
8+pairs+vs+8+pairs
1.40E&03
4
40
Single&housed+(SH)+males+vs+SH+females
8+pairs+vs+8+pairs
1.55E&04
0
36
GH+males+vs+SH+males
8+pairs+vs+8+pairs
1
28
64
GH+females+vs+SH+females
8+pairs+vs+8+pairs
1.55E&04
0
36
No+Food+males+vs+No+Food+females
8+pairs+vs+8+pairs
0.025641
12
48
Food+males+vs+Food+females
12+pairs+vs+12+pairs
9.53E&04
15
3.304
No+Food+males+vs+Food+males
8+pairs+vs+12+pairs
0.047869
28
1.9785
No+Food+females+vs+Food+females
8+pairs+vs+12+pairs
4.97E&04
2.5
3.4823
No+Food+males+vs+No+Food+females
8+pairs+vs+8+pairs
2
32
68
Food+males+vs+Food+females
12+pairs+vs+12+pairs
1.64E&02
42
2.3993
No+Food+males+vs+Food+males
8+pairs+vs+12+pairs
0.047869
28
1.9785
No+Food+females+vs+Food+females
8+pairs+vs+12+pairs
2
48
&InF
No+Food+males+vs+No+Food+females
8+pairs+vs+8+pairs
0.025641
12
48
Food+males+vs+Food+females
12+pairs+vs+12+pairs
1.02E&05
0
4.4136
No+Food+males+vs+Food+males
8+pairs+vs+12+pairs
2
48
&InF
No+Food+females+vs+Food+females
8+pairs+vs+12+pairs
7.57E&03
13
2.6708
Figure&2
Comparisons&&(Group&1&(G1)&vs&Group&2&(G2))
N&number
Methods
p
&value
U&value
W&value
z&value
signedrank
tstat
df
sd
Eb5+>+GFP+vs+Eb5+>+CsChR+During+Stimulation+
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
1.45E&06
0
4.8182
Eb5+>+CsChR+Before+vs+During+Stimulation+
16+pairs
Wilcoxon+signed&rank+test
4.37E&04
&3.517
0
Eb5+>+GFP+vs+Eb5+>+CsChR+During+Stimulation+
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
5.18E&07
0
5.0197
Eb5+>+CsChR+Before+vs+During+Stimulation+
16+pairs
Wilcoxon+signed&rank+test
4.38E&04
&3.516
0
Eb5+>+GFP+vs+Eb5+>+CsChR+During+Stimulation+
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
1.98E&05
15
4.2671
Eb5+>+CsChR+Before+vs+During+Stimulation+
16+pairs
Wilcoxon+signed&rank+test
2.41E&04
&3.672
13.5
Eb5+>+GFP+vs+Eb5+>+CsChR+During+Stimulation+
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
2.30E&05
16
4.2335
Eb5+>+CsChR+Before+vs+During+Stimulation+
16+pairs+
Wilcoxon+signed&rank+test
7.83E&04
&3.359
19
Figure&3
Comparisons&&(Group&1&(G1)&vs&Group&2&(G2))
N&number
Methods
p
&value
U&value
W&value
z&value
signedrank
tstat
df
sd
(Male)+CAP+>+GFP+vs+CAP+>+CsChR+During+Stimulation+
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
2.90E&06
2
4.6777
(Male)+CAP+>+CsChR+Before+vs+During+Stimulation+
16+pairs
Wilcoxon+signed&rank+test
7.51E&04
&3.37
3
(Female)+CAP+>+GFP+vs+CAP+>+CsChR+During+Stimulation+
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
1.26E&06
0
4.8459
(Female)+CAP+>+CsChR+Before+vs+During+Stimulation+
16+pairs
Wilcoxon+signed&rank+test
2.63E&04
&3.007
10
(Male)+MAP+>+GFP+vs+MAP+>+CsChR+During+Stimulation+
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
0.71463
133.5
0.3657
(Male)+MAP+>+CsChR+Before+vs+During+Stimulation+
16+pairs
Wilcoxon+signed&rank+test
0.1942
74
(Male)+CAP+>+GFP+vs+CAP+>+CsChR+During+Stimulation+
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
1
240.5
0
(Male)+CAP+>+CsChR+Before+vs+During+Stimulation+
16+pairs
Wilcoxon+signed&rank+test
0.1789
1.3442
115
(Fly+vs+Magnet+target&Male+tester)+CAP+>+GFP+vs+CAP+>+CsChR+
12+flies+vs+12+flies
0.02623
35.5
2.2228
(Fly+vs+Magnet+target&Female+tester)+CAP+>+GFP+vs+CAP+>+CsChR+
8+flies+vs+8+flies
0.028594
11.5
47.5
(Male+vs+Female+target&Male+tester)+CAP+>+GFP+vs+CAP+>+CsChR+
15+flies+vs+15+flies
1.99E&03
39
3.0922
(Male+vs+Female+target&Female+tester)+CAP+>+GFP+vs+CAP+>+CsChR+
15+flies+vs+15+flies
2.73E&04
23
3.6399
(Male+vs+Female+target&Male+tester)+CAP+>+CsChR+vs+(Male+vs+Female+target&Female+tester)+CAP+>+CsChR
15+flies+vs+15+flies
1.30E&04
20
3.8262
(Female+vs+Female+target&Male+tester)+CAP+>+GFP+vs+CAP+>+CsChR+
16+flies+vs+16+flies
0.061547
78
1.8695
(Female+vs+Female+target&Female+tester)+CAP+>+GFP+vs+CAP+>+CsChR+
16+flies+vs+16+flies
0.39596
105
0.8489
(Female+vs+Female+target&Male+tester)+CAP+>+CsChR+vs+(Female+vs+Female+target&Female+tester)+CAP+>+CsChR
16+flies+vs+16+flies
0.16263
90.5
1.3963
(Male+vs+Female+target&Male+tester)+CAP+>+CsChR+vs+(Female+vs+Female+target&Male+tester)+CAP+>+CsChR
15+flies+vs+16+flies
0.03612
72
2.0956
(Male+vs+Female+target&Female+tester)+CAP+>+CsChR+vs+(Female+vs+Female+target&Female+tester)+CAP+>+CsChR
15+flies+vs+16+flies
2.43E&02
68
2.2522
(Male&Approach+>+attack)+CAP+>+GFP+vs+CAP+>Kir2.1
13+pairs+vs+16+pairs
9.22E&06
6
4.4347
(Male&Approach)+CAP+>+GFP+vs+CAP+>+Kir2.1
13+pairs+vs+16+pairs
1.02E&04
15
3.8853
(Male&Lunge)+CAP+>+GFP+vs+CAP+>+Kir2.1+
13+pairs+vs+16+pairs
9.19E&06
6
4.4353
(Female&Approach+>+attack)+CAP+>+GFP+vs+CAP+>Kir2.1
16+pairs+vs+16+pairs
4.43E&04
45.5
3.513
(Female&Approach)+CAP+>+GFP+vs+CAP+>+Kir2.1
16+pairs+vs+16+pairs
1.25E&04
28.5
3.737
(Female&Headbutt)+CAP+>+GFP+vs+CAP+>+Kir2.1+
16+pairs+vs+16+pairs
3.92E&04
33.5
3.5454
Figure&4
Comparisons&&(Group&1&(G1)&vs&Group&2&(G2))
N&number
Methods
p
&value
U&value
W&value
z&value
signedrank
tstat
df
sd
(Stim+1&Female&Approach)+CAP+>+GFP+vs+CAP+>+CsChR
16+pairs+vs+16+pairs
7.95E&05
29
3.946
(Stim+2&Female&Approach)+CAP+>+GFP+vs+CAP+>+CsChR
16+pairs+vs+16+pairs
5.88E&04
40.5
3.4372
(Stim+3&Female&Approach)+CAP+>+GFP+vs+CAP+>+CsChR
16+pairs+vs+16+pairs
9.33E&07
0
4.9053
(Stim+4&Female&Approach)+CAP+>+GFP+vs+CAP+>+CsChR
16+pairs+vs+16+pairs
1.38E&06
2.5
4.8285
(Stim+5&Female&Approach)+CAP+>+GFP+vs+CAP+>+CsChR
16+pairs+vs+16+pairs
2.08E&06
4
4.745
(Stim+1&Male&Approach)+CAP+>+GFP+vs+CAP+>+CsChR
15+pairs+vs+15+pairs
0.83118
113.5
0.2132
(Stim+2&Male&Approach)+CAP+>+GFP+vs+CAP+>+CsChR
15+pairs+vs+15+pairs
0.12267
76
1.5437
(Stim+3&Male&Approach)+CAP+>+GFP+vs+CAP+>+CsChR
15+pairs+vs+15+pairs
9.94E&03
50.5
2.578
(Stim+4&Male&Approach)+CAP+>+GFP+vs+CAP+>+CsChR
15+pairs+vs+15+pairs
2.98E&06
0.5
4.6723
(Stim+5&Male&Approach)+CAP+>+GFP+vs+CAP+>+CsChR
15+pairs+vs+15+pairs
3.00E&06
0
4.6711
(Stim+1&Female&Headbutt)+CAP+>+GFP+vs+CAP+>+CsChR
16+pairs+vs+16+pairs
0.14251
98
1.4665
(Stim+2&Female&Headbutt)+CAP+>+GFP+vs+CAP+>+CsChR
16+pairs+vs+16+pairs
0.1213
90.5
1.5493
(Stim+3&Female&Headbutt)+CAP+>+GFP+vs+CAP+>+CsChR
16+pairs+vs+16+pairs
0.018327
33.5
3.741
(Stim+4&Female&Headbutt)+CAP+>+GFP+vs+CAP+>+CsChR
16+pairs+vs+16+pairs
3.03E&05
25.5
4.1713
(Stim+5&Female&Headbutt)+CAP+>+GFP+vs+CAP+>+CsChR
16+pairs+vs+16+pairs
1.01E&06
8
4.8896
(Stim+1&Male&Lunge)+CAP+>+GFP+vs+CAP+>+CsChR
15+pairs+vs+15+pairs
2
112
&InF
(Stim+2&Male&Lunge)+CAP+>+GFP+vs+CAP+>+CsChR
15+pairs+vs+15+pairs
2
112.5
&InF
(Stim+3&Male&Lunge)+CAP+>+GFP+vs+CAP+>+CsChR
15+pairs+vs+15+pairs
2
112.5
&InF
(Stim+4&Male&Lunge)+CAP+>+GFP+vs+CAP+>+CsChR
15+pairs+vs+15+pairs
2
112.5
&InF
(Stim+5&Male&Lunge)+CAP+>+GFP+vs+CAP+>+CsChR
15+pairs+vs+15+pairs
2
112
&InF
Male+mean+∆F/F+vs+Female+mean+∆F/F
15+flies++vs+15+flies
Mann&Whitney+U&test
0.033998
68
1.825
24.109
Male+Pre&stim+vs+Stim+mean+∆F/F
15+flies
Wilcoxon+signed&rank+test
6.10E&05
0
Female+Pre&stim+vs+Stim+mean+∆F/F
15+flies
Wilcoxon+signed&rank+test
6.10E&05
0
(Chrimson)+Male+intensity+vs+Female+intensity+
15+flies+vs+15+flies
Mann&Whitney+U&test
0.58974
99
0.5392
24.109
(GCaMP)+Male+intensity+vs+Female+intensity
15+flies+vs+15+flies
Mann&Whitney+U&test
0.34009
89
0.954
24.109
Figure&5
Comparisons&&(Group&1&(G1)&vs&Group&2&(G2))
N&number
Methods
p
&value
U&value
W&value
z&value
signedrank
tstat
df
sd
(Male)+CAP+>+GFP+vs+CAP+>+CsChR+During+Stimulation+
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
0.089633
97.5
1.6973
(Male)+CAP+>+CsChR+Before+vs+During+Stimulation+
16+pairs
Wilcoxon+signed&rank+test
0.25
0
(Male)+MAP+>+GFP+vs+MAP+>+CsChR+During+Stimulation+
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
1.48E&06
17
4.814
(Male)+MAP+>+CsChR+Before+vs+During+Stimulation+
16+pairs
Wilcoxon+signed&rank+test
1.22E&04
0
(Male&Approach+>+attack)+MAP+>+GFP+vs+MAP+>Kir2.1
24+pairs+vs+24+pairs
1.49E&03
141
3.1773
(Male&Approach)+MAP+>+GFP+vs+MAP+>+Kir2.1
24+pairs+vs+24+pairs
0.67226
267
0.4231
(Male&Lunge)+MAP+>+GFP+vs+MAP+>+Kir2.1+
24+pairs+vs+24+pairs
0.005147
168.5
2.5658
(Approach>attack)+CAP+MAP+>+ChR+&+Empty+driver+>+Kir2.1+vs++CAP+MAP+>+ChR+&+MAP+>+Kir2.1+
16+pairs+vs+16+pairs
1.12E&06
0
4.87
(Approach)+CAP+MAP+>+ChR+&+Empty+driver+>+Kir2.1+vs++CAP+MAP+>+ChR+&+MAP+>+Kir2.1+
16+pairs+vs+16+pairs
0.057944
77.5
1.8961
(Lunge)+CAP+MAP+>+ChR+&+Empty+driver+>+Kir2.1+vs++CAP+MAP+>+ChR+&+MAP+>+Kir2.1+
16+pairs+vs+16+pairs
3.60E&06
6
4.633
(Female)+CAP+>+GFP+vs+CAP+>+CsChR+During+Stimulation+
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
1.12E&06
0
4.87
(Female)+CAP+>+CsChR+Before+vs+During+Stimulation+
16+pairs
Wilcoxon+signed&rank+test
1.22E&04
0
Figure&6
Comparisons&&(Group&1&(G1)&vs&Group&2&(G2))
N&number
Methods
p
&value
U&value
W&value
z&value
signedrank
tstat
df
sd
MAP+mean+∆F/F+Pre&stim+vs+Stim
10+flies
Wilcoxon+signed&rank+test
2.00E&03
0
MAP+mean+∆F/F+Stim+vs+fpC1+mean+∆F/F+Stim
10+flies+vs+8flies
Mann&Whitney+U&test
0.006728
9
2.71
11.255
6Bii
fpC1+mean+∆F/F+Pre&stim+vs+Stim
8+flies
Wilcoxon+signed&rank+test
1.80E&03
0
fpC1+>+Chrimson+(26E01Gal4+++dsxFlp+++FlpOFF+cassette)+++++Pre&stim+vs+Stim
16+pairs+vs+16+pairs
Wilcoxon+signed&rank+test
1
22.5
fpC1+>+STOP+(BDPG4U+++dsxFlp+++FlpOFF+cassette)+stim+vs+fpC1+>+Chrimson+(26E01Gal4+++dsxFlp+++FlpOFF+
cassette)+Stim
8+pairs+vs+16+pairs
Mann&Whitney+U&test
0.20325
85
1.2723
fpC1+>+Chrimson+(26E01Gal4+++dsxFlp+++FlpOFF+cassette)+++++Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
0.0078
2.5
fpC1+>+STOP+(BDPG4U+++dsxFlp+++FlpOFF+cassette)+stim+vs++fpC1+>+Chrimson+(26E01Gal4+++dsxFlp+++FlpOFF+
cassette)+Stim
8+pairs+vs+16+pairs
Mann&Whitney+U&test
0.028589
31.5
2.1891
(Approach+>+attack)+fpC1+>+GFP+vs+fpC1+>+Kir2.1
8+pairs+vs+12+pairs
4.40E&04
4
3.515
(Approach)+fpC1+>+GFP+vs+fpC1+>+Kir2.1
8+pairs+vs+12+pairs
0.12398
33
1.5383
(Headbutt)+fpC1+>+GFP+vs+fpC1+>+Kir2.1
8+pairs+vs+12+pairs
2.52E&04
2
3.6598
(Approach+>+attack)+Ctrl+vs+fpC1+>+Kir2.1
16+pairs+vs+16+pairs
2.45E&04
24
3.6669
(Approach)+Ctrl+vs+fpC1+>+Kir2.1
16+pairs+vs+16+pairs
0.009466
49.5
2.5947
(Headbutt)+Ctrl+vs+fpC1+>+Kir2.1
16+pairs+vs+16+pairs
0.001528
35.5
3.1692
Figure&7
Comparisons&&(Group&1&(G1)&vs&Group&2&(G2))
N&number
Methods
p
&value
U&value
W&value
z&value
signedrank
tstat
df
sd
(MAP)+GH+mean+∆F/F+Pre&stim+vs+Stim
14+flies
Wilcoxon+signed&rank+test
0.583
43
(MAP)+SH+mean+∆F/F+Pre&stim+vs+Stim
14+flies
Wilcoxon+signed&rank+test
1.22E&04
0
(MAP)+GH+mean+∆F/F+stim+vs+SH+mean+∆F/F+stim
14+flies+vs+14+flies
Mann&Whitney+U&test
0.005439
37
2.7798
21.764
(fpC1)+GH+mean+∆F/F+Pre&stim+vs+Stim
16+flies
Wilcoxon+signed&rank+test
4.38E&04
&3.516
6D
7Ai
1Bi
Mann&Whitney+U&test+
Mann&Whitney+U&test+
1Bii
Mann&Whitney+U&test
1Biii
3Aiii
Mann&Whitney+U&test+
Mann&Whitney+U&test+
5D
3Bi
Mann&Whitney+U&test+
Mann&Whitney+U&test+
Mann&Whitney+U&test+
Mann&Whitney+U&test+
3Bii
5Ai
5Aii
3Ci
3Cii
3Biii
6Cii
Mann&Whitney+U&test+
Mann&Whitney+U&test+
4Ai
4Aii
5C
4Aiii
4Aiv
4Bii
Mann&Whitney+U&test+
Mann&Whitney+U&test+
Mann&Whitney+U&test+
Mann&Whitney+U&test+
1Ci
Mann&Whitney+U&test
Mann&Whitney+U&test+
Mann&Whitney+U&test+
1Cii
1Ciii
3Aii
2Ci
2Cii
2Ciii
2Civ
3Ai
6Ci
4Bi
Mann&Whitney+U&test
7Aii
6E
6Aii
5B
(fpC1)+SH+mean+∆F/F+Pre&stim+vs+Stim
16+flies
Wilcoxon+signed&rank+test
6.10E&05
0
(fpC1)+GH+mean+∆F/F+stim+vs+SH+mean+∆F/F+stim
16+flies+vs+16+flies
Mann&Whitney+U&test
4.43E&03
52
2.8455
(Approach+/+GH
GH)+CAP+>+GFP+vs+CAP+>+CsChrimson
16+pairs+vs+16+pairs
Mann&Whitney+U&test
0.055295
79
1.9166
(Approach+/+GH
SH)+CAP+>+GFP+vs+CAP+>+CsChrimson
16+pairs+vs+20+pairs
Mann&Whitney+U&test
4.50E&06
17
4.5867
(Approach+/+GH
SH)+CAP+>+CsChrimson+vs+No+light+control
20+pairs+vs+20+pairs+
Mann&Whitney+U&test
9.71E&03
104
2.5859
(Approach)+CAP+>+CsChrimson+GH
GH+vs+GH
SH+
16+pairs+vs+20+pairs+
Mann&Whitney+U&test
4.39E&05
32
4.086
(Lunge+/+GH
GH)+CAP+>+GFP+vs+CAP+>+CsChrimson
16+pairs+vs+16+pairs
2
128
&InF
(Lunge+/+GH
SH)+CAP+>+GFP+vs+CAP+>+CsChrimson
16+pairs+vs+20+pairs
0.075269
121
1.7788
(Lunge+/+GH
SH)+CAP+>+CsChrimson+vs+No+light+control
20+pairs+vs+20+pairs+
0.090535
165
1.3375
(Lunge)+CAP+>+CsChrimson+GH
GH+vs+GH
SH+
16+pairs+vs+20+pairs+
0.01264
112
2.3332
(Approach+/+GH
GH)+CAP+>+GFP+vs+CAP+>+CsChrimson
16+pairs+vs+15+pairs
0.001385
40
3.1978
(Approach+/+GH
SH)+CAP+>+GFP+vs+CAP+>+CsChrimson
16+pairs+vs+16+pairs
6.23E&06
11.5
4.5184
(Approach+/+GH
SH)+CAP+>+CsChrimson+vs+No+light+control
16+pairs+vs+16+pairs
9.04E&05
28.5
3.915
(Approach)+CAP+>+CsChrimson+GH
GH+vs+GH
SH+
15+pairs+vs+16+pairs
0.010924
67
2.3093
(Headbutt+/+GH
GH)+CAP+>+GFP+vs+CAP+>+CsChrimson
16+pairs+vs+15+pairs
0.91885
118
0.1019
(Headbutt+/+GH
SH)+CAP+>+GFP+vs+CAP+>+CsChrimson
16+pairs+vs+16+pairs
0.001478
58
3.1789
(Headbutt+/+GH
SH)+CAP+>+CsChrimson+vs+No+light+control
16+pairs+vs+16+pairs
0.009518
70
2.5929
(Headbutt)+CAP+>+CsChrimson+GH
GH+vs+GH
SH+
15+pairs+vs+16+pairs
0.003082
57.5
2.9594
Figure'S1
Comparisons''(Group'1'(G1)'vs'Group'2'(G2))
N'number
Methods
p
'value
U'value
W'value
z&value
ranksum
tstat
df
sd
Peak+Value+of+angular+velocity+Male+vs+Female+approach
125+bouts+vs+191+bouts
Mann&Whitney+U&test+
0.6677
10943
0.4293
Distance+to+other+at+the+beginning+of+the+bouts++Male+vs+Female
125+bouts+vs+191+bouts
Mann&Whitney+U&test+
0.42908
11309
0.7908
Distance+to+other+at+the+end+of+the+bouts++Male+vs+Female
125+bouts+vs+191+bouts
Mann&Whitney+U&test+
0.11992
10702
1.5551
Facing+angle+at+the+beginning+of+the+bouts+Male+vs+Female
125+bouts+vs+191+bouts
Mann&Whitney+U&test+
0.055465
10416
1.9152
Facing+angle+at+theend+of+the+bouts+Male+vs+Female
125+bouts+vs+191+bouts
Mann&Whitney+U&test+
0.24057
11005
1.1736
Peak+Value+of+acceleration+Male+vs+Female+approach
125+bouts+vs+191+bouts
Mann&Whitney+U&test+
0.84072
10039
0.201
Bout+duration+Male+vs+Female
125+bouts+vs+191+bouts
Mann&Whitney+U&test+
0
10179
10.306
Peak+Value+of+angular+velocity+Male+vs+Female+approach
197+bouts+vs+230+bouts
Mann&Whitney+U&test+
0.31229
21370
1.0104
Distance+to+other+at+the+beginning+of+the+bouts++Male+vs+Female
197+bouts+vs+230+bouts
Mann&Whitney+U&test+
0.059701
19593
1.883
Distance+to+other+at+the+end+of+the+bouts++Male+vs+Female
197+bouts+vs+230+bouts
Mann&Whitney+U&test+
0.12348
21884
1.5403
Facing+angle+at+the+beginning+of+the+bouts+Male+vs+Female
197+bouts+vs+230+bouts
Mann&Whitney+U&test+
0.22468
21111
1.2142
Facing+angle+at+theend+of+the+bouts+Male+vs+Female
197+bouts+vs+230+bouts
Mann&Whitney+U&test+
0.31683
21382
1.001
Peak+Value+of+acceleration+Male+vs+Female+approach
197+bouts+vs+230+bouts
Mann&Whitney+U&test+
0.12827
20721
1.521
Bout+duration+Male+vs+Female
197+bouts+vs+230+bouts
Mann&Whitney+U&test+
0.4163
21622
0.8129
Duration+Approach+vs+headbutt
191+bouts+vs+394+bouts
Mann&Whitney+U&test+
2.71E&106
21.898
93590
Distance+to+other+at+the+beginning+of+the+bouts++Approach+vs+headbutt
191+bouts+vs+394+bouts
Mann&Whitney+U&test+
1.35E&67
17.372
89265
Facing+angle+at+the+beginning+of+the+bouts+Approach+vs+headbutt
191+bouts+vs+394+bouts
Mann&Whitney+U&test+
4.62E&32
11.786
78557
(Precision)+Male+vs+Female
40+flies+vs+40+flies
Mann&Whitney+U&test+
0.76737
769
0.2958
(Recall)+Male+vs+Female
40+flies+vs+40+flies
Mann&Whitney+U&test+
0.93843
791.5
0.0772
Figure&S2
Comparisons&&(Group&1&(G1)&vs&Group&2&(G2))
N&number
Methods
p
&value
U&value
W&value
z&value
signedrank
tstat
df
sd
(Male/Approach)+BDP+>+CsChR+stim+vs+R60G08+>+CsChR++stim
16+pairs+vs+15+pairs
Mann&Whitney+U&test+
4.20E&05
16.5
4.0963
(Male/Approach)+R60G08+>+CsChR+Pre&stim+vs+Stim
15+pairs
Wilcoxon+signed&rank+test
0.00105
13
(Male/Lunge)+BDP+>+CsChR+stim+vs+R60G08+>+CsChR++stim
16+pairs+vs+15+pairs
Mann&Whitney+U&test+
3.66E&07
0
5.0857
(Male/Lunge)+R60G08+>+CsChR+Pre&stim+vs+Stim
15+pairs
Wilcoxon+signed&rank+test
6.10E&05
0
(Female/Approach)+BDP+>+CsChR+stim+vs+R60G08+>+CsChR++stim
13+pairs+vs+16+pairs
Mann&Whitney+U&test+
5.28E&06
0
4.5534
(Female/Approach)+R60G08+>+CsChR+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
4.16E&04
&3.53
0
(Female/Headbutt)+BDP+>+CsChR+stim+vs+R60G08+>+CsChR++stim
13+pairs+vs+16+pairs
Mann&Whitney+U&test+
4.78E&06
0
4.5744
(Female/Headbutt)+R60G08+>+CsChR+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
4.36E&04
&3.517
0
(Male)+R60G08&Gal4+++Flp&ON+cassette+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
0.25
9
(Male)+R60G08&Gal4+++otd&Flp+++Flp&ON+cassette+Pre&stim+vs+Stim
17+pairs
Wilcoxon+signed&rank+test
2.91E&04
&3.623
0
(Male)+R60G08&Gal4+++Flp&OFF+cassette+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
4.37E&04
&3.517
0
(Male)+R60G08&Gal4+++otd&Flp+++Flp&OFF+cassette+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
0.25
0
(Male)+R60G08&Gal4+++Flp&ON+cassette++Stim+vs+R60G08&Gal4+++otd&Flp+++Flp&ON+cassette+Stim
16+pairs+vs+17+pairs
Mann&Whitney+U&test+
4.71E&07
0
5.0379
(Male)+R60G08&Gal4+++Flp&OFF+cassette++Stim+vs+R60G08&Gal4+++otd&Flp+++Flp&OFF+cassette+Stim
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
6.54E&07
0
4.9746
(Male)+R60G08&Gal4+++otd&Flp+++Flp&ON+cassette++Stim+vs+R60G08&Gal4+++otd&Flp+++Flp&OFF+cassette+Stim
17+pairs+vs+16+pairs
Mann&Whitney+U&test+
4.71E&07
0
5.0379
(Female)+R60G08&Gal4+++Flp&ON+cassette+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
0.123
50.5
(Female)+R60G08&Gal4+++otd&Flp+++Flp&ON+cassette+Pre&stim+vs+Stim
24+pairs
Wilcoxon+signed&rank+test
1.81E&05
&4.288
0
(Female)+R60G08&Gal4+++Flp&OFF+cassette+Pre&stim+vs+Stim
24+pairs
Wilcoxon+signed&rank+test
1.81E&05
&4.288
0
(Female)+R60G08&Gal4+++otd&Flp+++Flp&OFF+cassette+Pre&stim+vs+Stim
24+pairs
Wilcoxon+signed&rank+test
0.3594
25
(Female)+R60G08&Gal4+++Flp&ON+cassette++Stim+vs+R60G08&Gal4+++otd&Flp+++Flp&ON+cassette+Stim
16+pairs+vs+24+pairs
Mann&Whitney+U&test+
9.68E&08
0
5.3326
(Female)+R60G08&Gal4+++Flp&OFF+cassette++Stim+vs+R60G08&Gal4+++otd&Flp+++Flp&OFF+cassette+Stim
24+pairs+vs+24+pairs
Mann&Whitney+U&test+
9.13E&10
0
6.1239
(Female)+R60G08&Gal4+++otd&Flp+++Flp&ON+cassette++Stim+vs+R60G08&Gal4+++otd&Flp+++Flp&OFF+cassette+Stim
24+pairs+vs+24+pairs
Mann&Whitney+U&test+
9.15E&10
0
6.1235
Figure&S3
Comparisons&&(Group&1&(G1)&vs&Group&2&(G2))
N&number
Methods
p
&value
U&value
W&value
z&value
signedrank
tstat
df
sd
(Male)+R60G08+neurons+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
4.36E&04
&3.517
0
(Male)+Eb5+neurons+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
4.38E&04
&3.516
0
(Male)+R60G08+/Eb5&+neurons+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
0.0156
13
(Male)+R60G08+neurons+Stim+vs+R60G08+/Eb5&+neurons+Stim
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
1.52E&06
0
4.8084
(Female)+R60G08+neurons+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
6.10E&05
0
(Female)+Eb5+neurons+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
4.30E&04
&3.521
0
(Female)+R60G08+/Eb5&+neurons+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
0.4375
6
(Female)+R60G08+neurons+Stim+vs+R60G08+/Eb5&+neurons+Stim
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
2.94E&06
1.5
4.6752
Figure&S4
Comparisons&&(Group&1&(G1)&vs&Group&2&(G2))
N&number
Methods
p
&value
U&value
W&value
z&value
signedrank
tstat
df
sd
(Female/Approach)+MAP+>+GFP+Stim+vs+MAP+>+CsChR+Stim
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
0.54
111.5
0.6128
(Female/Approach)+MAP+>+CsChR+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
0.6931
59.5
(Female/Headbutt)+MAP+>+GFP+vs+MAP+>+CsChR
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
0.15745
92
1.4137
(Female/Headutt)+MAP+>+CsChR+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
0.5515
54.5
(Female)+fpC1+driver+++Flp&ON+cassette+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
0.1997
&1.283
(Female)+fpC1+driver+++otd&Flp+++Flp&ON+cassette+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
3.83E&07
&5.077
(Female)+fpC1+driver+++Flp&OFF+cassette+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
7.54E&06
&4.478
(Female)+fpC1+driver+++otd&Flp+++Flp&OFF+cassette+Pre&stim+vs+Stim
16+pairs
Wilcoxon+signed&rank+test
0.526373
0.6336
(Female)+fpC1+driver+++Flp&ON+cassette++Stim+vs+fpC1+driver+++otd&Flp+++Flp&ON+cassette+Stim
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
7.74E&07
0
4.9417
(Female)+fpC1+driver+++Flp&OFF+cassette++Stim+vs+fpC1+driver+++otd&Flp+++Flp&OFF+cassette+Stim
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
1.79E&06
9
4.776
(Female)+fpC1+driver+++otd&Flp+++Flp&ON+cassette++Stim+vs+fpC1+driver+++otd&Flp+++Flp&OFF+cassette+Stim
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
3.83E&07
0
5.0774
S4F
MAP>GCaMP7b+vs+fpC1>GCaMP7b
10+flies+vs+10+flies
Mann&Whitney+U&test+
0.96985
49
0.0378
13.229
Figure&S5
Comparisons&&(Group&1&(G1)&vs&Group&2&(G2))
N&number
Methods
p
&value
U&value
W&value
z&value
signedrank
tstat
df
sd
(Male&to&Male&Aggression)&Pre1stim&vs&Stim
8&pairs
Wilcoxon&signed1rank&test
0.0078
0
(Male&to&Female&Aggression)&Pre1stim&vs&Stim
8&pairs
Wilcoxon&signed1rank&test
1
0
(30s&Baseline&/&Male&vs&Female&target1Male&tester)&CAP&>&GFP&vs&CAP&>&CsChR&
15&flies&vs&15&flies
Mann1Whitney&U1test&
1.99E103
39
3.0922
(30s&Baseline&/&Male&vs&Female&target1Female&tester)&CAP&>&GFP&vs&CAP&>&CsChR&
15&flies&vs&15&flies
Mann1Whitney&U1test&
2.73E104
23
3.6399
(30s&Baseline&/&Male&vs&Female&target1Male&tester)&CAP&>&CsChR&vs&(Male&vs&Female&target1Female&tester)&CAP&>&CsChR
15&flies&vs&15&flies
Mann1Whitney&U1test&
1.30E104
20
3.8262
(2MIN&Baseline&/&Male&vs&Female&Target&1&Male&tester)&CAP>GFP&vs&CAP>CsCR&
16&flies&vs&16&flies
Mann1Whitney&U1test&
3.42E103
50
2.9271
(2MIN&Baseline&/&Male&vs&Female&Target&1&Female&tester)&CAP>GFP&vs&CAP>CsCR&
16&flies&vs&16&flies
Mann1Whitney&U1test&
4.59E104
31
3.5036
(2MIN&Baseline&/&Male&vs&Female&Target&1&Male&tester)&CAP>CsCR&vs&(Female&tester)&CAP>CsCR&
16&flies&vs&16&flies
Mann1Whitney&U1test&
0.010281
66
2.3401
(30s&Baseline&/&Male&vs&Female&Target&1&Male&tester)&CAP>GFP&vs&(2MIN)&CAP>GFP&
15&flies&vs&16&flies
Mann1Whitney&U1test&
0.54348
104.5
0.60756
(30s&Baseline&/&Male&vs&Female&Target&1&Male&tester)&CAP>CsCR&vs&(2MIN)&CAP>CsCR&
15&flies&vs&16&flies
Mann1Whitney&U1test&
0.6774
109
0.41601
(30s&Baseline&/&Male&vs&Female&Target&1&Female&tester)&CAP>GFP&vs&(2MIN)&CAP>GFP
15&flies&vs&16&flies
Mann1Whitney&U1test&
1
111.5
0
(30s&Baseline&/&Male&vs&Female&Target&1&Female&tester)&CAP>CsCR&vs&(2MIN)&CAP>CsCR&
15&flies&vs&16&flies
Mann1Whitney&U1test&
0.87389
115.5
0.15872
S5Ci
(Mean+Velocity+/Male)+CAP+>+GFP+vs+CAP+>+Kir2.1+
13+pairs+vs+16+pairs
Mann&Whitney+U&test+
0.1571
325
1.415
S5Cii
(Mean+Velocity+/Female)+CAP+>+GFP+vs+CAP+>+Kir2.2
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
0.0967
572
1.6609
S5Ciii
(Mean+Velocity+/Male)+MAP+>+GFP+vs+MAP+>+Kir2.3
24+pairs+vs+24+pairs
Mann&Whitney+U&test+
0.4795
1055
0.7071
S5Civ
(Mean+Velocity+/Male)+fpC1+>+GFP+vs+fpC1+>+Kir2.4
8+pairs+vs+12+pairs
Mann&Whitney+U&test+
0.8357
215
0.2074
Figure&S6
Comparisons&&(Group&1&(G1)&vs&Group&2&(G2))
N&number
Methods
p
&value
U&value
W&value
z&value
signedrank
tstat
df
sd
(CAP/Male)+GH+Pre&stim+vs+Stim
15+flies
Wilcoxon+signed&rank+test
6.10E&05
0
(CAP/Male)+SH+Pre&stim+vs+Stim
15+flies
Wilcoxon+signed&rank+test
6.10E&05
0
(CAP/Male)+GH+Stim+vs+SH+Stim
15+flies+vs+15+flies
Mann&Whitney+U&test+
0.4553
94
0.7466
24.109
(CAP/Female)+GH+Pre&stim+vs+Stim
16+flies
Wilcoxon+signed&rank+test
4.38E&04
0
(CAP/Female)+SH+Pre&stim+vs+Stim
16+flies
Wilcoxon+signed&rank+test
2.93E&04
0
(CAP/Female)+GH+Stim+vs+SH+Stim
16+flies+vs+16+flies
Mann&Whitney+U&test+
0.50954
110
0.6596
26.533
(CAP)+GH+male+stim+vs+GH+female+stim
15+flies+vs+16+flies
Mann&Whitney+U&test+
0.012071
56
2.5101
25.298
(CAP)+SH+male+stim+vs+SH+female+stim
15+flies+vs+16+flies
Mann&Whitney+U&test+
0.040388
70
1.9567
25.298
(MAP/Male)+GH+Pre&stim+vs+Stim
10+flies
Wilcoxon+signed&rank+test
2.00E&03
0
(MAP/Male)+SH+Pre&stim+vs+Stim
10+flies
Wilcoxon+signed&rank+test
2.00E&03
0
(MAP/Male)+GH+Stim+vs+SH+Stim
10+flies+vs+10+flies
Mann&Whitney+U&test+
0.79134
46
0.2646
13.229
(fpC1/Female)+GH+Pre&stim+vs+Stim
15+flies
Wilcoxon+signed&rank+test
6.10E&05
0
(fpC1/Female)+SH+Pre&stim+vs+Stim
17+flies
Wilcoxon+signed&rank+test
2.93E&04
&3.621
0
(fpC1/Female)+GH+Stim+vs+SH+Stim
15+flies+vs+17+flies
Mann&Whitney+U&test+
0.2127
94
1.2462
26.481
Figure&S7
Comparisons&&(Group&1&(G1)&vs&Group&2&(G2))
N&number
Methods
p
&value
U&value
W&value
z&value
signedrank
tstat
df
sd
(Approach+to+attack)+Eb5+>+Chrimson+++Empty+>+Kir2.1+vs+Eb5+>+Chrimson+++tk+>+Kir2.1
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
4.46E&04
37
3.5111
(Approach)+Eb5+>+Chrimson+++Empty+>+Kir2.1+vs+Eb5+>+Chrimson+++tk+>+Kir2.1
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
0.7755
120
0.2852
(Lunge)+Eb5+>+Chrimson+++Empty+>+Kir2.1+vs+Eb5+>+Chrimson+++tk+>+Kir2.1
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
7.19E&07
0.5
4.9561
(Approach+to+attack)+tk+>+Chrimson+++Empty+>+Kir2.1+vs+tk+>+Chrimson+++Eb5+>+Kir2.1
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
0.605384
114
0.5167
(Approach)+tk+>+Chrimson+++Empty+>+Kir2.1+vs+tk+>+Chrimson+++Eb5+>+Kir2.1
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
0.0674
79.5
1.8288
(Lunge)+tk+>+Chrimson+++Empty+>+Kir2.1+vs+tk+>+Chrimson+++Eb5+>+Kir2.1
16+pairs+vs+16+pairs
Mann&Whitney+U&test+
0.1843
92.5
1.3277
S5A
S5B
Mann&Whitney+U&test
7Bii
S2Cii
7Ci
Mann&Whitney+U&test
7Bi
S7B
7Cii
Mann&Whitney+U&test
S4Aii
S3Bii
S2Ai
S2Aii
S7A
S2Aiii
S1Aii
S1B
S1C
S1D
S2Ci
S2Aiv
S4Eii
S6A
S6B
S6C
S3Bi
7Aii