Published June 1977
| Version Published
Journal Article
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Detection of a contaminant cell culture line by restriction endonuclease cleavage patterns of mitochondrial DNA
Abstract
A putative HeLa cell culture line was discovered to be contaminated with mouse cells by examination of agarose gel profiles of restriction endonuclease digests of mitochondrial DNA. The contamination was confirmed by karyotypic analysis, and by observation of the mouse satellite band in an analytical buoyant density centrifugation of total cellular DNA. Restriction endonuclease analysis of mitochondrial DNA is suggested as a useful method for monitoring the species of cells in culture.
Additional Information
© 1977 Oxford University Press. Dedicated to the memory of Professor Jerome Vinograd. Received February 10, 1977. We are grateful to Mrs. Paula Berry, City of Hope National Medical Center, for help with the karyotype preparations. This work was supported by grants GM 21704 from NIH at Wayne State University and GM 15327 and CA 08014 from NIH at Caltech. This is contribution No. 5501 from the Division of Chemistry and Chemical Engineering.Attached Files
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Additional details
Identifiers
- PMCID
- PMC343754
- Eprint ID
- 2026
- Resolver ID
- CaltechAUTHORS:GROnar77
Funding
- NIH
- GM 21704
- NIH
- GM 15327-09
- NIH
- CA 08014
Dates
- Created
-
2006-03-02Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field
Caltech Custom Metadata
- Other Numbering System Name
- Caltech Division of Chemistry and Chemical Engineering
- Other Numbering System Identifier
- 5501