B.
A.
D.
C.
E.
G.
F.
I.
H.
J.
With
initiator
probes,
No
amplifier
hairpins
Without
initiator
probes,
With B1
amplifier
hairpins
DAPI
ANME-2b
Seep-SRB1g
B1
Composite
nifH
Supplementary Figure 8.
Representative images for the negative control experiments for visualizing mRNA expression by HCR-FISH.
(A-E) Images of ANME-2b/Seep-1g aggregates in the DAPI, TRITC, FITC and cy5 channels as well as the composite of images from al
l the
channels. These images were from an experiment without nifH-targeted initiator probes but with just B1 fluorescent hairpins in
order to visualize
potential non-specific binding of B1 hairpins. (F-J) These images were from an experiment with nifH-targeted initiator probes
but without B1
fluorescent hairpins in order to visualize the background fluoresence in the the samples.