Correlation between ANME-2b and Seep-1g
fluorescent signal
Correlation between Seep-1g and nifH
fluorescent signal
Correlation between ANME-2b and nifH
fluorescent signal
Pearson’s correlation coefficient,
PC=0.165
,
p-value-100%, Costes’ method
Pearson’s correlation coefficient,
PC=0.541
,
p-value=100%, Costes method
Pearson’s correlation coefficient,
PC=0.521
,
p-value =Costes’ method
Manders’ correlation coefficients, after
thresholding,
M1=0.022, M2=0.008
Manders’ correlation coefficients, after
thresholding,
M1=0.891, M2=0.163
Manders’ correlation coefficients, after
thresholding,
M1=0.958, M2=0.39
a. Scatterplots of pixel intensities
HCR-FISH control experiment: With initiator probes, No amplifier hairpins
ANME-2b
Seep-1g
nifH
DAPI
P
C
=0.16
5
,
P
C
=0.54
1
,
P
C
=0.52
1
,
Supplementary Figure 10.
Colocalization analysis of HCR-FISH control experiment with initiator probes and without amplifier hairpins. ANME-2b is
stained in the cy3 channel, Seep-1g in the FITC channel. No dyes fluorescing in the cy5 channel are present in this experiment
a. Scatterplots of pixel
intensities of the FITC, cy3 and cy5 channel suggest there is some bleed through in both the cy3 and FITC channels.Neither the
Pearson’s correlation
coefficient which measures covariance between the channels in proportion to their standard deviation nor Manders’ correlation c
oefficients M1 and M2,
which better correct for differences in intensity, are high enough to indicate significant cross-correlation.