Correlation between ANME-2b and Seep-1g
fluorescent signal
Correlation between Seep-1g and nifH
fluorescent signal
Correlation between ANME-2b and nifH
fluorescent signal
Pearson’s correlation coefficient,
PC=0.666
,
p-value-100%, Costes’ method
Pearson’s correlation coefficient,
PC=0.332
,
p-value=100%, Costes method
Pearson’s correlation coefficient,
PC=0.267
,
p-value =Costes’ method
Manders’ correlation coefficients, after
thresholding,
M1=0.801, M2=0.601
Manders’ correlation coefficients, after
thresholding,
M1=0.002, M2=0.127
Manders’ correlation coefficients, after
thresholding,
M1=0.02, M2=0.221
a. Scatterplots of pixel intensities
HCR-FISH control experiment: Without initiator probes, with amplifier hairpins
DAPI
ANME-2b
Seep-SRB1g
B1
PC
=0.66
6
,
P
C
=0.33
2
,
,
P
C
=0.267
,
Supplementary Figure 11.
Colocalization analysis of HCR-FISH control experiment without initiator probes and with B1 amplifier hairpins.
A
NME-2b is stained in the cy3 channel, Seep-1g in the FITC channel and the B1 amplifiers, without the initiator probes are visua
lized in the
cy5 channel a. Scatterplots of pixel intensities of the FITC, cy3 and cy5 channel suggest there is some bleed through in both t
he cy3 and FITC
channels. Similar to the no amplifier control, neither the Pearson’s correlation coefficient nor M1 and M2 are high enough to i
ndicate
significant cross-correlation
.