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Published October 1983 | public
Journal Article

Identification of two distinct regulatory regions adjacent to the human β-interferon gene


To study the regulation of the human β-interferon (β-IFN) gene by poly(I)-poly(C), we analyzed the expression of deletion mutants of the cloned gene introduced into mouse cells on a new bovine papilloma virus (BPV) vector. In stable cell lines transformed by a BPV-IFN plasmid containing the β-IFN structural gene with 210 bp of DNA to the 5′ side of its mRNA cap site (denoted −210), human β-IFN mRNA is induced ∼400-fold by poly(I)-poly(C), and reproducible levels of expression are observed for independent cell lines. Our studies indicate that there are two distinct regulatory regions adjacent to the gene, located between −77 and −19, and between −210 and −107. The −77 to −19 region is required for constitutive and induced IFN gene expression, and both are drastically reduced by deletion to −73. When sequences between −210 and −107 are deleted, the constitutive level of IFN gene expression is increased 5- to 10-fold, while induced expression is essentially unaffected. Deletion of the −210 to −107 region also alters the kinetics of induction of the gene.

Additional Information

© 1983 MIT. Received July 11, 1983; revised August 8, 1983. We thank Michael Green, Pamela Mellon, Peter Hawley, David Kimelman, and the members of the Maniatis and Melton laboratories for helpful discussions, Tadatsugu Taniguchi for the mouse IFN cDNA clone, and Sophia Stern for help in preparing the manuscript. K. Z. was supported by a National Institutes of Health training grant. D. D. is a Fellow of the Jane Coffin Childs Memorial Fund for Medical Research. This work was supported by grants from the National Institutes of Health and the National Science Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

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October 18, 2023