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Published July 25, 2017 | public
Journal Article Open

Characterizing Single Polymeric and Protein Nanoparticles with Surface Plasmon Resonance Imaging Measurements


Near-infrared surface plasmon resonance imaging (SPRI) microscopy is used to detect and characterize the adsorption of single polymeric and protein nanoparticles (PPNPs) onto chemically modified gold thin films in real time. The single-nanoparticle SPRI responses, Δ%R_(NP), from several hundred adsorbed nanoparticles are collected in a single SPRI adsorption measurement. Analysis of Δ%R_(NP) frequency distribution histograms is used to provide information on the size, material content, and interparticle interactions of the PPNPs. Examples include the measurement of log-normal Δ%R_(NP) distributions for mixtures of polystyrene nanoparticles, the quantitation of bioaffinity uptake into and aggregation of porous NIPAm-based (N-isopropylacrylamide) hydrogel nanoparticles specifically engineered to bind peptides and proteins, and the characterization of the negative single-nanoparticle SPRI response and log-normal Δ%R_(NP) distributions obtained for three different types of genetically encoded gas-filled protein nanostructures derived from bacteria.

Additional Information

© 2017 American Chemical Society. Received: June 1, 2017; Accepted: July 10, 2017; Published: July 10, 2017. This work was supported by the National Science Foundation through grant CHE-1403506 (R.M.C.), the National Institutes of Health through grants R01-GM059622 (R.M.C.) and R01-EB018975 (M.G.S.), and the Heritage Medical Research Institute (M.G.S.). DLS data were acquired at the Laser Spectroscopy Facility in the Department of Chemistry at UCI. The authors acknowledge Y. Terada for the synthesis of p-acrylamidophenyl-α-d-mannopyranoside. The authors declare no competing financial interests.


The version of this paper that was published ASAP July 12, 2017, contained an error in eq 1. The corrected version was reposted July 13, 2017.

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August 19, 2023
August 19, 2023