Structural Features and Domain Organization of Mutant Huntingtin
SUPPLEMENTAL INFORMATION
FIGURE S1.
CD spectra of
spin labeled derivatives of HDx1
-
thioredoxin fusion proteins prior to
enzymatic cleavage of the fusion tag. T
he CD spectra are color coded to indicate the location
within the HDx1 sequence where spin labels were introduced
.
The specific labeled sites are given
within each panel
.
Spectra were recorded at protein concentrations of 5
μ
M.
Structural Features and Domain Organization of Mutant Huntingtin
FIGURE S2
.
CD spectra of
HDx1 fibrils. Spectra were obtained
at protein concentrations of 30
μ
M. The CD spectra are color coded to indicate the location within the HDx1 sequence where
spin labels were introduced. The top panel contains representative derivatives that were spin
la
beled in the N
-
terminus. The middle and bottom panels contain spectra from HDx1 labeled at
representative sites in the polyQ and C
-
terminal domain
s, respectively
.