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Published July 2018 | Published
Journal Article Open

Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy


Optical-resolution photoacoustic microscopy (OR-PAM) has demonstrated fast, label-free volumetric imaging of optical-absorption contrast within the quasiballistic regime of photon scattering. However, the limited numerical aperture of the ultrasonic transducer restricts the detectability of the photoacoustic waves, thus resulting in incomplete reconstructed features. To tackle the limited-view problem, we added an oblique illumination beam to the original coaxial optical-acoustic scheme to provide a complementary detection view. The virtual augmentation of the detection view was validated through numerical simulations and tissue-phantom experiments. More importantly, the combination of top and oblique illumination successfully imaged a mouse brain in vivo down to 1 mm in depth, showing detailed brain vasculature. Of special note, it clearly revealed the diving vessels that were long missing in images from original OR-PAM.

Additional Information

© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE). Paper 180145R received: 9 March 2018; accepted: 8 June 2018; published: 6 July 2018. The authors appreciate Professor James Ballard's kind help with editing the article. L.W. has a financial interest in Microphotoacoustics, Inc., CalPACT, LLC, and Union Photoacoustic Technologies, Ltd., which, however, did not support this work. This work was supported in part by National Institutes of Health grants DP1 EB016986 (NIH Director's Pioneer Award), R01 CA186567 (NIH Director's Transformative Research Award), R01 EB016963, U01 NS090579 (BRAIN Initiative), and U01 NS099717 (BRAIN Initiative).

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