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Published January 1988 | public
Journal Article

Nucleotide sequence of dengue 2 RNA and comparison of the encoded proteins with those of other flaviviruses


We have determined the complete sequence of the RNA of dengue 2 virus (S1 candidate vaccine strain derived from the PR-159 isolate) with the exception of about 15 nucleotides at the 5' end. The genome organization is the same as that deduced earlier for other flaviviruses and the amino acid sequences of the encoded dengue 2 proteins show striking homology to those of other flaviviruses. The overall amino acid sequence similarity between dengue 2 and yellow fever virus is 44.7%, whereas that between dengue 2 and West Nile virus is 50.7%. These viruses represent three different serological subgroups of mosquito-borne flaviviruses. Comparison of the amino acid sequences shows that amino acid sequence homology is not uniformly distributed among the proteins; highest homology is found in some domains of nonstructural protein NS5 and lowest homology in the hydrophobic polypeptides ns2a and 2b. In general the structural proteins are less well conserved than the nonstructural proteins. Hydrophobicity profiles, however, are remarkably similar throughout the translated region. Comparison of the dengue 2 PR-159 sequence to partial sequence data from dengue 4 and another strain of dengue 2 virus reveals amino acid sequence homologies of about 64 and 96%, respectively, in the structural protein region. Thus as a general rule for flaviviruses examined to date, members of different serological subgroups demonstrate 50% or less amino acid sequence homology, members of the same subgroup average 65-75% homology, and strains of the same virus demonstrate greater than 95% amino acid sequence similarity.

Additional Information

© 1988 Academic Press. Inc. Received July 13, 1987; accepted September 2, 1987. We are grateful to E. M. Lenches for excellent technical assistance, to C. S. Hahn for helpful discussions, and to P. Wright and D. Trent for furnishing us with results prior to publication. This work was supported by Grants AI-20612 and AI-10793 from NIH, Grant DMB 86-17372 from NSF, by a contract from the U.S. Army Medical Development Command, and by a grant from the World Health Organization. R.G. was supported by a fellowship from the National Research Council of Brazil (CNPq).

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