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Published September 15, 1983 | public
Journal Article Open

RNA transcripts for I-J polypeptides are apparently not encoded between the I-A and I-E subregions of the murine major histocompatibility complex


The I-J subregion of the mouse major histocompatibility complex has been reported to encode antigenic determinants expressed by suppressor T cells. Previously, cosmid clones were obtained from mouse sperm DNA that contain all of the sequences between the I-A and I-E subregions, where I-J has been mapped genetically. However, hybridization of these sequences to RNA prepared from several I-J-positive suppressor T-cell hybridomas did not reveal the presence of a transcript. In addition, no rearrangements in this DNA were detected in the suppressor T cells that we have analyzed. Our results indicate that the I-J polypeptides are not encoded between the I-A and I-E subregions of the major histocompatibility complex. We discuss several hypotheses concerning the possible location and expression of I-J genes.

Additional Information

© 1983 by the National Academy of Sciences. Contributed by Leroy Hood, June 6, 1983. We thank Dr. Donal Murphy for many helpful discussions. This work was supported by National Institutes of Health Research Grants AI 17565, AI 18959, AI 15353, by Public Health Service Grant number IF32CA06693 awarded by the National Cancer Inst., Department of Health and Human Services (E.K.), and by a grant from the Ministry of Education, Culture, and Science of Japan. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.


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