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Published December 1, 2012 | Accepted Version + Published
Journal Article Open

Wide and scalable field-of-view Talbot-grid-based fluorescence microscopy

Abstract

Here we report a low-cost and simple wide field-of-view (FOV) on-chip fluorescence-imaging platform, termed fluorescence Talbot microscopy (FTM), which utilizes the Talbot self-imaging effect to enable efficient fluorescence imaging over a large and directly scalable FOV. The FTM prototype has a resolution of 1.2 μm and an FOV of 3.9  mm×3.5  mm. We demonstrate the imaging capability of FTM on fluorescently labeled breast cancer cells (SK-BR-3) and human embryonic kidney 293 (HEK) cells expressing green fluorescent protein.

Additional Information

© 2012 Optical Society of America. Received September 18, 2012; revised October 24, 2012; accepted November 5, 2012. We thank Danielle V. Bower (Biological Imaging Center, Caltech) for the help in sample preparation. This project is funded by NIH under the grant 1R01AI096226-01.

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Published - ol-37-23-5018.pdf

Accepted Version - nihms491195.pdf

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