Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published December 7, 2006 | Supplemental Material
Journal Article Open

Odorant Receptor Map in the Mouse Olfactory Bulb: In Vivo Sensitivity and Specificity of Receptor-Defined Glomeruli


Odorant identity is represented in the olfactory bulb (OB) by the glomerular activity pattern, which reflects a combination of activated odorant receptors (ORs) in the olfactory epithelium. To elucidate this neuronal circuit at the molecular level, we established a functional OR identification strategy based on glomerular activity by combining in vivo Ca^(2+) imaging, retrograde dye labeling, and single-cell RT-PCR. Spatial and functional mapping of OR-defined glomeruli revealed that the glomerular positional relationship varied considerably between individual animals, resulting in different OR maps in the OB. Notably, OR-defined glomeruli exhibited different ligand spectra and far higher sensitivity compared to the in vitro pharmacological properties of corresponding ORs. Moreover, we found that the olfactory mucus was an important factor in the regulation of in vivo odorant responsiveness. Our results provide a methodology to examine in vivo glomerular responses at the receptor level and further help address the long-standing issues of olfactory sensitivity and specificity under physiological conditions.

Additional Information

© 2006 Elsevier. Received: May 25, 2006. Revised: October 5, 2006. Accepted: October 19, 2006. Published: December 6, 2006. We thank T. Hasegawa Co. Ltd. for odorant compounds, members of K. Mori's lab for advice on the medetomidine anesthesia protocol, N. Uchida for technical advice on olfactory bulb surgery, and Touhara's lab members for support. This work was supported in part by grants from the Program for Promotion of Basic Research Activities for Innovative Biosciences, Japan (PROBRAIN) (to K.T.), Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (to Y.Y. and K.T.), the Japan Society for the Promotion of Science (JSPS) (to Y.O.), and Regional Research and Development Consortium Project (to K.T.).

Attached Files

Supplemental Material - mmc1.pdf


Files (464.0 kB)
Name Size Download all
464.0 kB Preview Download

Additional details

August 19, 2023
October 25, 2023