Supplementary Fig 1 Sex
-
specific alternative splicing of
Drosophila transformer
(
tra
) and
double sex
(
dsx
) genes.
The female
-
specific intron between
tra
exons 1 and 2
(
traF
) is spliced out entirely in females,
while in males some sequence remains, producing a stop codon and resulting in a premature termination of
the TRA protein
44
. Inversely, the male
-
specific intron be
tween
dsx
exons 3 and 5 (
dsxM
) is spliced out in
males, while in females only its small part is spliced out and an entire exon 4 carrying a premature stop
codon remains
45
. To establish female
-
or male
-
specific expression of antibiotic
-
resistance genes, we
inserted a
traF
or
dsxM
intron sequence into the coding sequence of
PuroR
and
NeoR
.