Published May 1976
| Version Published
Journal Article
Open
Translation of murine leukemia virus RNA in cell-free systems from animal cells
Abstract
The virion RNA of Moloney murine leukemia virus (MuLV) has been translated in eukaryotic cell-free systems derived from mouse L- and human HeLa cells. In both systems at least three polypeptides, approximately 60,000, 70,000, and 180,000 in apparent molecular weight, were formed in response to the added 35S MuLV RNA. All three polypeptides were precipitable with antiserum to detergent-disrupted MuLV. Fingerprint analysis of tryptic digests indicated that all three contain anino acid sequences in common with each other and with the major methionine-containing structural proteins of the virion.
Additional Information
© 1976 American Society for Microbiology. Received for publication 8 December 1975 This work was supported by an American Cancer Society Eleanor Roosevelt Cancer Research Fellowship to I.M.K., by U.S. Public Health Service International Fellowship F0-5-TW-2135 to U.O., and by Public Health Service grants CA 14051 and AI 08814 from the National Cancer Institute and from the National Institute of Allergy and Infectious Diseases, respectively. D.B. and H.F.L. were recipients of grants VC-4G and NP-180 from the American Cancer Society. D.B. is an American Cancer Society Professor, and H.F.L. is the recipient of N.I.H. Research Career Development award 5-K04-GM50175.Attached Files
Published - KERjvir76.pdf
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Additional details
Identifiers
- PMCID
- PMC515589
- Eprint ID
- 6283
- Resolver ID
- CaltechAUTHORS:KERjvir76
Funding
- American Cancer Society
- VC-4G
- NIH Postdoctoral Fellowship
- F0-5-TW-2135
- NIH
- CA-14051
- NIH
- AI-08814
- American Cancer Society
- NP-180
- NIH
- 5-K04-GM50175
Dates
- Created
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2006-11-30Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field