In vivo dark-field reflection-mode photoacoustic microscopy
Reflection-mode photoacoustic microscopy with dark-field laser pulse illumination and high-numerical-aperture ultrasonic detection is designed and implemented in noninvasively imaged blood vessels in the skin in vivo. Dark-field optical illumination minimizes the interference caused by strong photoacoustic signals from superficial structures. A high-numerical-aperture acoustic lens provides high lateral resolution, 45–120μm in this system. A broadband ultrasonic detection system provides high axial resolution, estimated to be ∼15μm. The optical illumination and ultrasonic detection are in a coaxial confocal configuration for optimal image quality. The system is capable of imaging optical-absorption contrast as deep as 3mm in biological tissue.
Additional Information© 2005 Optical Society of America. Received October 4, 2004. This project was sponsored by National Institutes of Health grants R01 EB000712 and R01 NS46214.
Published - ol-30-6-625.pdf