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Published January 2018 | Supplemental Material + Accepted Version
Journal Article Open

High-resolution deep functional imaging of the whole mouse brain by photoacoustic computed tomography in vivo


Photoacoustic computed tomography (PACT) is a non-invasive imaging technique offering high contrast, high resolution, and deep penetration in biological tissues. We report a PACT system equipped with a high frequency linear transducer array for mapping the microvascular network of a whole mouse brain with the skull intact and studying its hemodynamic activities. The linear array was scanned in the coronal plane to collect data from different angles, and full-view images were synthesized from the limited-view images in which vessels were only partially revealed. We investigated spontaneous neural activities in the deep brain by monitoring the concentration of hemoglobin in the blood vessels and observed strong interhemispherical correlations between several chosen functional regions, both in the cortical layer and in the deep regions. We also studied neural activities during an epileptic seizure and observed the epileptic wave spreading around the injection site and the wave propagating in the opposite hemisphere.

Additional Information

© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Version of record online: 21 June 2017; Manuscript Accepted: 3 May 2017; Manuscript Revised: 2 May 2017; Manuscript Received: 29 January 2017. This work was funded by the National Institutes of Health (NIH) Grants U01 NS090579 (NIH Brain Initiative Award), U01 NS099717 (NIH Brain Initiative Award), R01 EB016963, DP1 EB016986 (Director's Pioneer Award), R01 CA186567 (Director's Transformative Research Award), S10 RR026922. The authors thank Prof. James Ballard for his close reading of the manuscript. L. V. Wang has a financial interest in Microphotoacoustics, Inc., which, however, did not support this work.

Attached Files

Accepted Version - nihms905372.pdf

Supplemental Material - jbio201700024-sup-0001-Movie.avi


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