1
Supp
orting
Information for
Single tissue proteomics in
Caenorhabditis elegans
reveals proteins
resident in intestinal lysosome
-
related organelles
.
Chieh
-
Hsiang Tan
1*
, Ting
-
Yu Wang
2*
, Heenam Park
1
, Brett Lomenick
2
, Tsui
-
Fen
Chou
1,2#
, and
Paul W. Sternberg
1#
1
: Division of Biology and Biological Engineering, California Institute of Technology.
2
: Proteome Exploration Laboratory, Beckman Institute, California Institute of Technology
.
*
denotes equal contribution;
#
for correspondence
Tsui
-
Fen Chou
Email:
tfchou@caltech.edu
Paul W. Sternberg.
Email: pws@caltech.edu
This PDF file
includes:
Figures
S1
to
S
2
Legends
for
Datasets
S1 to S
7
Other
supp
orting
materials
for this manuscript
include
the following
:
Datasets S1 to S
7
2
Fig. S1.
Tissue extracted from a single animal is sufficient for proteomic analysis. In an
independent trial, a total of 3991 proteins were identified from 3 samples of single
gonadal
tissues, with up to 3405 (85.3%) identifiable in a single gonad (A); a total of 2684 proteins were
identified from 3 samples of single intestinal tissues, with up to 2351 (87.6%) identifiable in a
single intestinal tissue (B). The Euler diagrams ar
e area
-
proportional. Lists of proteins described
in this figure can be found in SI Appendix
,
D
ataset S3.
3
Fig. S2
.
Number of samples in which each protein was quantifiable in the mass spectrometry
analysis described in Fig. 3A
-
E. A total of 3203
proteins were quantifiable from 6 samples of
gonadal tissue. A total of 3084 proteins were quantifiable from 6 samples of intestinal tissue. (
SI
Appendix
,
D
ataset S4).
4
Dataset
S1 (separate file)
.
Data associated with Fig. 2.
Dataset
S
2
(separate file)
.
Data associated with Fig. 3
.
Dataset
S
3
(separate file)
.
Data associated with Fig. S1
Dataset
S
4
(separate file)
.
Data associated with Fig. S2
Dataset
S
5
(separate file)
.
Data associated with Fig. 4
Dataset
S
6
(separate file)
.
Data associated with Fig. 5
Dataset
S
7
(separate file)
.
All analyzed proteomic data used in this study. Including data from
every trial and including the protein abundance, normalized and without normalization.