Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published June 2012 | Published
Journal Article Open

Quantitative photoacoustic microscopy of optical absorption coefficients from acoustic spectra in the optical diffusive regime


Photoacoustic (PA) microscopy (PAM) can image optical absorption contrast with ultrasonic spatial resolution in the optical diffusive regime. Conventionally, accurate quantification in PAM requires knowledge of the optical fluence attenuation, acoustic pressure attenuation, and detection bandwidth. We circumvent this requirement by quantifying the optical absorption coefficients from the acoustic spectra of PA signals acquired at multiple optical wavelengths. With the acoustic spectral method, the absorption coefficients of an oxygenated bovine blood phantom at 560, 565, 570, and 575 nm were quantified with errors of <3%. We also quantified the total hemoglobin concentration and hemoglobin oxygen saturation in a live mouse. Compared with the conventional amplitude method, the acoustic spectral method provides greater quantification accuracy in the optical diffusive regime. The limitations of the acoustic spectral method was also discussed.

Additional Information

© 2012 SPIE. Paper 11795 received Dec. 28, 2011; revised manuscript received Mar. 30, 2012; accepted for publication Apr. 12, 2012; published online Jun. 5, 2012. We would like to thank Yan Liu, Dr. Xiao Xu, and Dr. Konstantin Maslov for useful discussions and Prof. James Ballard for editing the manuscript. This work was sponsored in part by the National Institutes of Health grants R01 EB008085, R01 EB000712, R01 CA134539, R01 CA157277, and U54 CA 136398. L. W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

Attached Files

Published - JBO_17_6_066011.pdf


Files (2.7 MB)
Name Size Download all
2.7 MB Preview Download

Additional details

August 22, 2023
October 20, 2023