A signal cascade originated from epidermis defines apical-basal patterning of Arabidopsis shoot apical meristems
Abstract
In multicellular organisms, a long-standing question is how spatial patterns of distinct cell types are initiated and maintained during continuous cell division and proliferation. Along the vertical axis of plant shoot apical meristems (SAMs), stem cells are located at the top while cells specifying the stem cells are located more basally, forming a robust apical-basal pattern. We previously found that in Arabidopsis SAMs, the HAIRY MERISTEM (HAM) family transcription factors form a concentration gradient from the epidermis to the interior cell layers, and this gradient is essential for the stem cell specification and the apical-basal patterning of the SAMs. Here, we uncover that epidermis specific transcription factors, ARABIDOPSIS THALIANA MERISTEM LAYER 1 (ATML1) and its close homolog, define the concentration gradient of HAM in the SAM through activating a group of microRNAs. This study provides a molecular framework linking the epidermis-derived signal to the stem cell homeostasis in plants.
Additional Information
© 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Received 20 March 2019; Accepted 11 February 2020; Published 05 March 2020. We thank the Purdue Bindley Bioscience Imaging Facility for access to the ZEISS LSM880 Laser scanning confocal microscope. We appreciate the generous support and help from Dr. Elliot Meyerowitz. We also thank Dr. Eric Mjolsness for the helpful discussions and suggestions on the parameter tests in the computational model. The work was supported by Purdue University start-up package and funds from Purdue Center for Plant Biology to Y.Z. Data availability: All data are available from the corresponding author upon request. The source data underlying Figs. 2b–l, 3b–i, 4b, c, e, f, 5a–c, 7i–l, and Supplementary Figs. 2, 1, 4, 10, 11, and 17 are provided as a Source Data file. Code availability: The code is available from the corresponding author upon request. Author Contributions: H.H., X.L., and Y.Z. conceived the research direction, H.H., L.L., X.L., and Y.Z. performed the experiments, A.Y. performed the model simulation, A.Y. and B.F. analyzed the sensitivity of model parameters, Yf.Z. contributed reagents and commented on the results, H.H., A.Y., X.L., and Y.Z. wrote the manuscript. All the authors read and agreed to the manuscript. The authors declare no competing interests.Attached Files
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Additional details
- PMCID
- PMC7058014
- Eprint ID
- 101814
- Resolver ID
- CaltechAUTHORS:20200310-085927238
- Purdue University
- Created
-
2020-03-10Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field