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Published July 28, 2020 | Submitted
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Enabling out-of-clinic human immunity studies via single-cell profiling of capillary blood

Abstract

An individual's immune system is driven by both genetic and environmental factors that vary over time. To better understand the temporal and inter-individual variability of gene expression within distinct immune cell types, we developed a platform that leverages multiplexed single-cell sequencing and out-of-clinic capillary blood extraction to enable simplified, cost-effective profiling of the human immune system across people and time at single-cell resolution. Using the platform, we detect widespread differences in cell type-specific gene expression between subjects that are stable over multiple days.

Additional Information

The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license. Posted July 26, 2020. The authors have declared no competing interest. Data Availability: Gene expression matrix and relevant metadata are available on https://data.caltech.edu/records/1407. Genome and FASTQ files are not being released to protect the identity of the subjects. Code Availability: Custom code made for diurnal and subject specific gene detection is available on https://github.com/thomsonlab/capblood-seq.

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Submitted - 2020.07.25.210468v1.full.pdf

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August 19, 2023
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March 5, 2024