Published July 28, 2020
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Enabling out-of-clinic human immunity studies via single-cell profiling of capillary blood
Abstract
An individual's immune system is driven by both genetic and environmental factors that vary over time. To better understand the temporal and inter-individual variability of gene expression within distinct immune cell types, we developed a platform that leverages multiplexed single-cell sequencing and out-of-clinic capillary blood extraction to enable simplified, cost-effective profiling of the human immune system across people and time at single-cell resolution. Using the platform, we detect widespread differences in cell type-specific gene expression between subjects that are stable over multiple days.
Additional Information
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license. Posted July 26, 2020. The authors have declared no competing interest. Data Availability: Gene expression matrix and relevant metadata are available on https://data.caltech.edu/records/1407. Genome and FASTQ files are not being released to protect the identity of the subjects. Code Availability: Custom code made for diurnal and subject specific gene detection is available on https://github.com/thomsonlab/capblood-seq.Attached Files
Submitted - 2020.07.25.210468v1.full.pdf
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2020.07.25.210468v1.full.pdf
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Additional details
- Eprint ID
- 104585
- Resolver ID
- CaltechAUTHORS:20200727-093309639
- Created
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2020-07-28Created from EPrint's datestamp field
- Updated
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2023-09-08Created from EPrint's last_modified field
- Caltech groups
- Division of Biology and Biological Engineering